Titanium (IV) ion-modified covalent organic frameworks for specific enrichment of phosphopeptides

To date, plenty of new alternative materials for phosphopeptides enrichment prior to mass spectrometry (MS) analysis appear, especially immobilized metal ion affinity chromatography (IMAC) materials. The variable combinations with different metal ions, chelating ligands and solid supports offer full...

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Bibliographic Details
Published in:Talanta (Oxford) 2017-05, Vol.166, p.133-140
Main Authors: Wang, Heping, Jiao, Fenglong, Gao, Fangyuan, Lv, Yayao, Wu, Qiong, Zhao, Yan, Shen, Yehua, Zhang, Yangjun, Qian, Xiaohong
Format: Article
Language:English
Subjects:
Covalent organic frameworks
Immobilized metal ion affinity chromatography
Phosphopeptide enrichment
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Summary:To date, plenty of new alternative materials for phosphopeptides enrichment prior to mass spectrometry (MS) analysis appear, especially immobilized metal ion affinity chromatography (IMAC) materials. The variable combinations with different metal ions, chelating ligands and solid supports offer full of optionality for IMAC. However, further improvement was predicted by the tedious and complex synthetic process. In this work, a novel covalent organic framework (COF)-based IMAC material (denoted TpPa-2-Ti4+) was prepared simply by direct immobilizing Ti (IV) into TpPa-2 COFs without any extra chelating ligands. The structure and composition of as-prepared composites were confirmed by PXRD, FT-IR and XPS, and a new flower-shaped Ti4+-IMAC with regular micro-nano hierarchical structure was observed in the SEM and TEM images. The obtained titanium (IV) ion-modified covalent organic frameworks demonstrated low limit of detection (4 fmol) and largely-satisfactory selectivity (β-casein: BSA=1:100) for phosphopeptide capturing from β-casein. Similarly, 18 and 17 phosphopeptides could be easily detected in the tryptic digest of α-casein or the digest mixture of α-casein and BSA (1:50). They were also successfully applied for enrichment of phosphopeptides from non-fat milk and HeLa cells with high sensitivity and satisfactory selectivity. All above results showed that the new titanium (IV) ion-modified covalent organic framework is expected to be a potential IMAC for phosphopeptide enrichment in large-scale phosphoproteomics studies. [Display omitted] •A novel covalent organic framework-based IMAC material was flexibly synthesized.•Titanium ions were directly immobilized in the nitrogen-rich frameworks of TpPa-2 COFs.•The enrichment performance was investigated and compared with commercial TiO2 materials.•All results provided high sensitivity and satisfactory selectivity for phosphopeptide enrichment.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2017.01.043