Gabapentin decreases microglial cells and reverses bilateral hyperalgesia and allodynia in rats with chronic myositis

In the present work, we investigated the antinociceptive effect of gabapentin in a chronic myositis model and its interference in spinal glial cells. Chronic myositis was induced by injection of Complete Freund Adjuvant (CFA) into the right gastrocnemius (GS) muscle of rats and tests for evaluating...

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Bibliographic Details
Published in:European journal of pharmacology 2017-03, Vol.799, p.111-117
Main Authors: Rosa, A.S., Freitas, M.F., Rocha, I.R.C., Chacur, M.
Format: Article
Language:English
Subjects:
Allodynia
Amines - pharmacology
Amines - therapeutic use
Animals
Astrocytes
Astrocytes - drug effects
Astrocytes - pathology
Behavior, Animal - drug effects
Cell Count
Chronic Disease
Chronic muscle pain
Cyclohexanecarboxylic Acids - pharmacology
Cyclohexanecarboxylic Acids - therapeutic use
Gabapentin
gamma-Aminobutyric Acid - pharmacology
gamma-Aminobutyric Acid - therapeutic use
Hyperalgesia
Hyperalgesia - complications
Hyperalgesia - drug therapy
Hyperalgesia - pathology
Male
Microglia
Microglia - drug effects
Microglia - pathology
Myalgia - complications
Myositis - complications
Rats
Rats, Wistar
Spinal Cord Dorsal Horn - pathology
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Summary:In the present work, we investigated the antinociceptive effect of gabapentin in a chronic myositis model and its interference in spinal glial cells. Chronic myositis was induced by injection of Complete Freund Adjuvant (CFA) into the right gastrocnemius (GS) muscle of rats and tests for evaluating mechanical hyperalgesia, thermal hyperalgesia and tactile allodynia were performed. Pharmacological treatment with gabapentin was administrated intrathecally and 100μg and 200μg doses were tested. For analyzing astrocytes and microglia in the spinal cord, immunochemistry assay was performed. It was found that gabapentin 200μg reverted CFA-induced chronic muscle pain bilaterally, in all applied tests and it was able to attenuate microglial but not astrocytes activation in the dorsal horn of spinal cord. In conclusion, gabapentin was able to inhibit hyperalgesia and allodynia in chronic myositis and also to attenuate spinal microglial activation. Therefore, gabapentin could be used as treatment for targeting chronic muscle pain.
ISSN:0014-2999
1879-0712
DOI:10.1016/j.ejphar.2017.02.012