Biotechnological applications of penicillin acylases: state-of-the-art

This review describes the most recent developments in the biotechnological applications of penicillin acylases. This group of enzymes is involved mainly in the industrial production of 6-aminopenicillanic acid and the synthesis of semisynthetic beta-lactam antibiotics. In addition, penicillin acylas...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2003, Vol.60 (5), p.507-514
Main Authors: ARROYO, M, DE LA MATA, I, ACEBAL, C, CASTILLON, M. Pilar
Format: Article
Language:English
Subjects:
Acids
Anti-Bacterial Agents - chemical synthesis
Antibiotics
Biocatalysts
Biological and medical sciences
Biotechnology
E coli
Enzyme engineering
Enzymes
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Industrial Microbiology
Industrial production
Methods. Procedures. Technologies
Microorganisms
Miscellaneous
Models, Molecular
Penicillanic Acid - analogs & derivatives
Penicillanic Acid - chemical synthesis
Penicillin
Penicillin Amidase - chemistry
Penicillin Amidase - metabolism
Peptides
Solvents
Studies
Technology, Pharmaceutical
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Summary:This review describes the most recent developments in the biotechnological applications of penicillin acylases. This group of enzymes is involved mainly in the industrial production of 6-aminopenicillanic acid and the synthesis of semisynthetic beta-lactam antibiotics. In addition, penicillin acylases can also be employed in other useful biotransformations, such as peptide synthesis and the resolution of racemic mixtures of chiral compounds. Particular emphasis is placed on advances in detection of new enzyme specificities towards other natural penicillins, enzyme immobilization, and optimization of enzyme-catalyzed hydrolysis and synthesis in the presence of organic solvents.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-002-1113-6