T Lymphocytes in Histiocytic Sarcomas of Flat-Coated Retriever Dogs

Flat-Coated Retriever dogs are predisposed to the development of histiocytic sarcoma (HS), a poorly differentiated, highly malignant neoplasm. The authors have previously documented a significant lymphocytic infiltrate in such tumors. The objective of this study was to examine the presence and expre...

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Bibliographic Details
Published in:Veterinary pathology 2017-07, Vol.54 (4), p.605-610
Main Authors: Marcinowska, A., Constantino-Casas, F., Williams, T., Hoather, T., Blacklaws, B., Dobson, J.
Format: Article
Language:English
Subjects:
Animals
CD3 Complex - immunology
CD79 Antigens - immunology
Dog Diseases - immunology
Dog Diseases - pathology
Dogs
Female
Forkhead Transcription Factors - immunology
Histiocytic Sarcoma - immunology
Histiocytic Sarcoma - pathology
Histiocytic Sarcoma - veterinary
Interleukin-2 Receptor alpha Subunit - immunology
Leukocyte Common Antigens - immunology
Male
T-Lymphocytes, Regulatory - pathology
Tumor Microenvironment - immunology
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Summary:Flat-Coated Retriever dogs are predisposed to the development of histiocytic sarcoma (HS), a poorly differentiated, highly malignant neoplasm. The authors have previously documented a significant lymphocytic infiltrate in such tumors. The objective of this study was to examine the presence and expression of regulatory T cells in HS tumor samples. Forty tumors were included in this study. All tumors were immunolabeled for CD3, CD79a, CD25, CD45RA, and FOXP3. The proportion of positive cells was compared between tumors presenting as a localized primary soft tissue mass (soft tissue origin HS) and disseminated HS affecting viscera, especially the spleen (splenic origin HS). By immunohistochemistry, 95% of infiltrating T cells were positive for Foxp3 in all sections, suggesting the presence of regulatory T cells. The proportion of cells positive for FOXP3 was higher in the tumors arising in soft tissues, whereas the proportion of CD45RA-positive cells was higher in the splenic origin HS. Canine HS has an aggressive clinical behavior and is uniformly fatal. The difference in the proportion of tumor-infiltrating lymphocytes positive for these 2 markers in the 2 locations may represent differences in tumor microenvironment between the 2 sites.
ISSN:0300-9858
1544-2217
DOI:10.1177/0300985817690208