Nitrite inhibition and limitation - the effect of nitrite spiking on anammox biofilm, suspended and granular biomass

Anaerobic ammonium oxidation (anammox) has been studied extensively while no widely accepted optimum values for nitrite (both a substance and inhibitor) has been determined. In the current paper, nitrite spiking (abruptly increasing nitrite concentration in reactor over 20 mg NO -NL ) effect on anam...

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Bibliographic Details
Published in:	Water science and technology 2017-01, Vol.75 (2), p.313-321
Main Authors:	Raudkivi, Markus, Zekker, Ivar, Rikmann, Ergo, Vabamäe, Priit, Kroon, Kristel, Tenno, Taavo
Format:	Article
Language:	eng
Subjects:	Ammonium Ammonium compounds Bacteria Batch reactors Biofilms Biomass Bioreactors Inhibition Microorganisms Nitrites Nitrogen removal Nucleotide sequence Oxidation PCR Polymerase chain reaction Reactors Removal rRNA 16S Sludge Spiking Thresholds Upflow anaerobic sludge blanket reactors
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Summary:	Anaerobic ammonium oxidation (anammox) has been studied extensively while no widely accepted optimum values for nitrite (both a substance and inhibitor) has been determined. In the current paper, nitrite spiking (abruptly increasing nitrite concentration in reactor over 20 mg NO -NL ) effect on anammox process was studied on three systems: a moving bed biofilm reactor (MBBR), a sequencing batch reactor (SBR) and an upflow anaerobic sludge blanket (UASB). The inhibition thresholds and concentrations causing 50% of biomass activity decrease (IC ) were determined in batch tests. The results showed spiked biomass to be less susceptible to nitrite inhibition. Although the values of inhibition threshold and IC concentrations were similar for non-spiked biomass (81 and 98 mg NO -NL , respectively, for SBR), nitrite spiking increased IC considerably (83 and 240 mg NO -NL , respectively, for UASB). As the highest total nitrogen removal rate was also measured at the aforementioned thresholds, there is basis to suggest stronger limiting effect of nitrite on anammox process than previously reported. The quantitative polymerase chain reaction analysis showed similar number of anammox 16S rRNA copies in all reactors, with the lowest quantity in SBR and the highest in MBBR (3.98 × 10 and 1.04 × 10 copies g TSS, respectively).
ISSN:	0273-1223 1996-9732