Delineating hierarchy of selenotranscriptome expression and their response to selenium status in chicken central nervous system

Selenium (Se) incorporated in selenoproteins as selenocysteine and supports various important cellular and organismal functions. We recently reported that chicken brain exhibited high priority for Se supply and retention under conditions of dietary Se deficiency and supernutrition Li et al. (2012) ....

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Published in:Journal of inorganic biochemistry 2017-04, Vol.169, p.13-22
Main Authors: Jiang, Xiu-Qing, Cao, Chang-Yu, Li, Zhao-Yang, Li, Wei, Zhang, Cong, Lin, Jia, Li, Xue-Nan, Li, Jing-Long
Format: Article
Language:English
Subjects:
Animals
Central nervous system
Central Nervous System - drug effects
Central Nervous System - metabolism
Cerebral Cortex - drug effects
Cerebral Cortex - metabolism
Chicken
Chickens
Hierarchy
Real-Time Polymerase Chain Reaction
Selenium
Selenium - metabolism
Selenium - pharmacology
Selenoproteins - genetics
Selenoproteome
Transcriptome - drug effects
Transcriptome - genetics
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Summary:Selenium (Se) incorporated in selenoproteins as selenocysteine and supports various important cellular and organismal functions. We recently reported that chicken brain exhibited high priority for Se supply and retention under conditions of dietary Se deficiency and supernutrition Li et al. (2012) . However, the selenotranscriptome expressions and their response to Se status in chicken central nervous system (CNS) are unclear. To better understand the relationship of Se homeostasis and selenoproteins expression in chicken CNS, 1day-old HyLine White chickens were fed a low Se diet (Se-L, 0.028mg/g) supplemented with 4 levels of dietary Se (0 to 5.0mgSe/kg) as Na2SeO3 for 8weeks. Then chickens were dissected for getting the CNS, which included cerebral cortex, cerebellum, thalamus, bulbus cinereus and marrow. The expressions of selenoproteome which have 24 selenoproteins were detected by the quantitative real-time PCR array. The concept of a selenoprotein hierarchy was developed and the hierarchy of different regions in chicken CNS was existence, especially cerebral cortex and bulbus cinereus. The expression of selenoproteins has a hierarch while changing Se content, and Selenoprotein T (Selt), Selenoprotein K (Selk), Selenoprotein W (Selw), Selenoprotein U (Selu), Glutathione peroxidase 3 (Gpx3), Glutathione peroxidase 4 (Gpx4), Selenoprotein P (Sepp1), Selenoprotein O (Selo), Selenoprotein 15 (Sel15), Selenoprotein N (Seln), Glutathione peroxidase 2 (Gpx2) and Selenoprotein P 2 (Sepp2) take more necessary function in the chicken CNS. Therefore, we hypothesize that hierarchy of regulated the transcriptions of selenoproteome makes an important role of CNS Se metabolism and transport in birds. Selenium involved in the synthesis of polypeptide in selenoprotein by selenocysteine. Selenium homeostasis is presented in chicken central nervous system (CNS) which fed the diets with low or supernutritional selenium. The hierarchy of regulated the transcriptions of selenoproteome played an important role of CNS selenium metabolism and transport. [Display omitted] •Selenoproteome has a hierarchy of expression in different Selenium (Se) levels.•A hierarchy of Se assignment is showed in chicken central nervous system (CNS).•The 12 selenoproteins are primary to modulate the allocation of Se in chicken CNS.•The cerebral cortex and bulbus cinereus have higher priority to utilize Se.•Selenotranscriptome hierarchy makes key roles of CNS Se metabolism and transport.
ISSN:0162-0134
1873-3344
DOI:10.1016/j.jinorgbio.2017.01.002