Kinetics of circulating progenitor cell mobilization during submaximal exercise

Circulating progenitor cells (CPCs) are a heterogeneous population of stem/progenitor cells in peripheral blood that includes hematopoietic stem and progenitor cells (HSPCs and HSCs), endothelial progenitor cells (EPCs), and mesenchymal stem cells (MSCs) that are involved in tissue repair and adapta...

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Published in:Journal of applied physiology (1985) 2017-03, Vol.122 (3), p.675-682
Main Authors: Niemiro, Grace M, Parel, Justin, Beals, Joseph, van Vliet, Stephan, Paluska, Scott A, Moore, Daniel R, Burd, Nicholas A, De Lisio, Michael
Format: Article
Language:English
Subjects:
Adult
Cell Movement - immunology
Chemokine CXCL12 - blood
Chemokine CXCL12 - immunology
Endothelial Progenitor Cells - cytology
Endothelial Progenitor Cells - immunology
Exercise - physiology
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - immunology
Humans
Kinetics
Male
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - immunology
Physical Exertion - immunology
Stem Cell Factor - blood
Stem Cell Factor - immunology
Stem Cells - cytology
Stem Cells - immunology
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Summary:Circulating progenitor cells (CPCs) are a heterogeneous population of stem/progenitor cells in peripheral blood that includes hematopoietic stem and progenitor cells (HSPCs and HSCs), endothelial progenitor cells (EPCs), and mesenchymal stem cells (MSCs) that are involved in tissue repair and adaptation. CPC mobilization during exercise remains uncharacterized in young adults. The purpose of this study was to investigate the kinetics of CPC mobilization during and after submaximal treadmill running and their relationship to mobilization factors. Seven men [age = 25.3 ± 2.4 yr, body mass index = 23.5 ± 1.0 kg/m , peak O uptake (V̇o ) = 60.9 ± 2.74 ml·kg ·min ] ran on a treadmill for 60 min at 70% V̇o Blood sampling occurred before (Pre), during [20 min (20e), 40 min (40e), 60 min (60e)], and after exercise [15 min (15p), 60 min (60p), 120 min (120p)] for quantification of CPCs (CD34 ), HSPCs (CD34 /CD45 ), HSCs (CD34 /CD45 /CD38 ), CD34 MSCs (CD45 /CD34 /CD31 /CD105 ), CD34 MSCs (CD45 /CD34 /CD31 /CD105 ), and EPCs (CD45 /CD34 /CD31 ) via flow cytometry. CPC concentration increased compared with Pre at 20e and 40e (2.7- and 2.4-fold, respectively, < 0.05). HSPCs and HSCs increased at 20e compared with 60p (2.7- and 2.8-fold, respectively, < 0.05), whereas EPCs and both MSC populations did not change. CXC chemokine ligand (CXCL) 12 (1.5-fold; < 0.05) and stem cell factor (1.3-fold; < 0.05) were increased at 40e and remained elevated postexercise. The peak increase in CPCs was positively correlated to concentration of endothelial cells during exercise with no relationship to CXCL12 and SCF. Our data show the kinetics of progenitor cell mobilization during exercise that could provide insight into cellular mediators of exercise-induced adaptations, and have implication for the use of exercise as an adjuvant therapy for CPC collection in hematopoietic stem cell transplant. Using a comprehensive evaluation of circulating progenitor cells (CPCs), we show that CPC mobilization during exercise is related to tissue damage, and not plasma concentrations of CXC chemokine ligand 12 and stem cell factor. These data have implications for the use of exercise interventions as adjuvant therapy for CPC mobilization in the context of hematopoietic stem cell transplant and also support the role of mobilized progenitor cells as cellular mediators of systemic adaptations to exercise.
ISSN:8750-7587
1522-1601
DOI:10.1152/japplphysiol.00936.2016