Diet-induced changes in hypothalamic pro-opio-melanocortin mRNA in the rat hypothalamus

Hypothalamic mRNA and peptide levels of pro-opio-melanocortin (POMC) and other neuropeptides were studied in rats that either develop obesity (diet-induced obese, DIO), when fed a palatable and hypercaloric diet (cafeteria diet, caf) or do not develop obesity (diet resistant, DR), when fed the same...

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Bibliographic Details
Published in:Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2002-06, Vol.23 (6), p.1063-1068
Main Authors: Torri, Carla, Pedrazzi, Patrizia, Leo, Giuseppina, Müller, Eugenio E, Cocchi, Daniela, Agnati, Luigi F, Zoli, Michele
Format: Article
Language:English
Subjects:
Animal Nutritional Physiological Phenomena
Animals
Cafeteria diet
Fats - pharmacology
High-fat diet
Hypothalamus - metabolism
In Situ Hybridization
Male
Neurons - metabolism
Obesity
Obesity - metabolism
Peptides - chemistry
Pro-opio-melanocortin
Pro-Opiomelanocortin - chemistry
Pro-Opiomelanocortin - metabolism
Rat
Rats
Rats, Sprague-Dawley
RNA - metabolism
RNA, Messenger - metabolism
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Summary:Hypothalamic mRNA and peptide levels of pro-opio-melanocortin (POMC) and other neuropeptides were studied in rats that either develop obesity (diet-induced obese, DIO), when fed a palatable and hypercaloric diet (cafeteria diet, caf) or do not develop obesity (diet resistant, DR), when fed the same diet. cafDIO rats showed a significant increase in POMC, but not in melanin concentrating hormone, mRNA levels as determined by semiquantitative in situ hybridization. cafDR and cafDIO rats showed no change in POMC-derived peptide levels, whereas neuropeptide Y immunoreactivity was significantly increased in cafDR rats. POMC mRNA levels were also studied in high-fat diet-fed rats but no significant change was observed. Altered hypothalamic transmission by POMC-derived peptides may contribute to the susceptibility of cafDIO rats to the weight promoting action of caf diet.
ISSN:0196-9781
1873-5169
DOI:10.1016/S0196-9781(02)00030-X