Gut content microbiota of introduced bigheaded carps (Hypophthalmichthys spp.) inhabiting the largest shallow lake in Central Europe

Studying the microbiota in the alimentary tract of bigheaded carps (Hypophthalmichthys spp.) gained special interest recently, as these types of investigations on non-native fish species may lead to a better understanding of their ecological role and feeding habits in an invaded habitat. For microbi...

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Bibliographic Details
Published in:Microbiological research 2017-01, Vol.195, p.40-50
Main Authors: Borsodi, Andrea K., Szabó, Attila, Krett, Gergely, Felföldi, Tamás, Specziár, András, Boros, Gergely
Format: Article
Language:English
Subjects:
Animals
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bigheaded carp
Carps - microbiology
Cluster Analysis
Denaturing Gradient Gel Electrophoresis
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Eukaryota - classification
Eukaryota - genetics
Eukaryota - isolation & purification
Fusobacteria
Gastrointestinal Microbiome
Gastrointestinal Tract - microbiology
Hungary
Hypophthalmichthys
Hypophthalmichthys spp
Intestinal microbiota
Lake Balaton
Lakes
Phylogeny
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
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Summary:Studying the microbiota in the alimentary tract of bigheaded carps (Hypophthalmichthys spp.) gained special interest recently, as these types of investigations on non-native fish species may lead to a better understanding of their ecological role and feeding habits in an invaded habitat. For microbiological examinations, bigheaded carp gut contents and water column samples from Lake Balaton (Hungary) were collected from spring to autumn in 2013. Denaturing Gradient Gel Electrophoresis (DGGE) and pyrosequencing of the 16S rRNA gene were performed to reveal the composition. According to the DGGE patterns, bacterial communities of water samples separated clearly from that of the intestines. Moreover, the bacterial communities in the foreguts and hindguts were also strikingly dissimilar. Based on pyrosequencing, both foregut and hindgut samples were predominated by the fermentative genus Cetobacterium (Fusobacteria). The presence of some phytoplankton taxa and the high relative abundance of cellulose-degrading bacteria in the guts suggest that intestinal microbes may have an important role in digesting algae and making them utilizable for bigheaded carps that lack cellulase enzyme. In turn, the complete absence of typical heterotrophic freshwater bacteria in all studied sections of the intestines indicated that bacterioplankton probably has a negligible role in the nutrition of bigheaded carps.
ISSN:0944-5013
1618-0623
DOI:10.1016/j.micres.2016.11.001