Tissue-Specific Induction of Hsp90 mRNA and Plasma Cortisol Response in Chinook Salmon following Heat Shock, Seawater Challenge, and Handling Challenge

In studying the whole-body response of chinook salmon (Oncorhynchus tshawytscha) to various stressors, we found that 5-hour exposure to elevated temperature (mean 21.6 degrees C; + 10.6 degrees C over ambient) induced a marked increase in Hsp90 messenger RNA accumulation in heart, brain, gill, muscl...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2000-07, Vol.2 (4), p.329-338
Main Authors: Palmisano, AN, Winton, JR, Dickhoff, WW
Format: Article
Language:English
Subjects:
Animal tissues
Brain
Cattle
Cellular biology
Cellular stress response
Cortisol
Fish
Freshwater fishes
Handling
Heart
Heat shock
High temperature
Hormones
Hsp90 protein
Kidneys
Liver
mRNA
Nucleic acids
Oncorhynchus tshawytscha
Plasma
Ribonucleic acid
RNA
Salmon
Seawater
Stress
Tissue
Tissues
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Summary:In studying the whole-body response of chinook salmon (Oncorhynchus tshawytscha) to various stressors, we found that 5-hour exposure to elevated temperature (mean 21.6 degrees C; + 10.6 degrees C over ambient) induced a marked increase in Hsp90 messenger RNA accumulation in heart, brain, gill, muscle, liver, kidney, and tail fin tissues. The most vital tissues (heart, brain, gill, and muscle) showed the greatest Hsp90-mRNA response, with heart tissue increasing approximately 35-fold. Heat shock induced no increase in plasma cortisol. In contrast, a standard handling challenge induced high plasma cortisol levels, but no elevation in Hsp90 mRNA in any tissue, clearly separating the physiological and cellular stress responses. We saw no increase either in tissue Hsp90 mRNA levels or in plasma cortisol concentrations after exposing the fish to seawater overnight.
ISSN:1436-2228
1436-2236
DOI:10.1007/s101260000005