Acclimation to salt modifies the activation of several osmotic stress-activated lipid signalling pathways in Chlamydomonas

Osmotic stress rapidly activates several phospholipid signalling pathways in the unicellular alga Chlamydomonas. In this report, we have studied the effects of salt-acclimation on growth and phospholipid signalling. Growing cells on media containing 100 mM NaCl increased their salt-tolerance but did...

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Bibliographic Details
Published in:Phytochemistry (Oxford) 2017-03, Vol.135, p.64-72
Main Authors: Meijer, Harold J.G., van Himbergen, John A.J., Musgrave, Alan, Munnik, Teun
Format: Article
Language:English
Subjects:
Acclimation
Acclimatization
Chlamydomonadaceae
Chlamydomonas - metabolism
Chlamydomonas moewusii
Diphosphates - analysis
Glycerol - analogs & derivatives
Glycerol - analysis
Green alga
Isomerism
Osmotic Pressure
Osmotic stress
Phosphatidic Acids
Phospholipase D - metabolism
Phospholipids
Plants - metabolism
Salt-Tolerance
Signal transduction
Signal Transduction - drug effects
Sodium Chloride - pharmacology
Sucrose - metabolism
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Summary:Osmotic stress rapidly activates several phospholipid signalling pathways in the unicellular alga Chlamydomonas. In this report, we have studied the effects of salt-acclimation on growth and phospholipid signalling. Growing cells on media containing 100 mM NaCl increased their salt-tolerance but did not affect the overall phospholipid content, except that levels of phosphatidylinositol phosphate (PIP) and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] were reduced by one-third. When these NaCl-acclimated cells were treated with increasing concentrations of salt, the same lipid signalling pathways as in non-acclimated cells were activated. This was witnessed as increases in phosphatidic acid (PA), lyso-phosphatidic acid (L-PA), diacylglycerol pyrophosphate (DGPP), PI(4,5)P2 and its isomer PI(3,5)P2. However, all dose-dependent responses were shifted to higher osmotic-stress levels, and the responses were lower than in non-acclimated cells. When NaCl-acclimated cells were treated with other osmotica, such as KCl and sucrose, the same effects were found, illustrating that they were due to hyperosmotic rather than hyperionic acclimation. The results indicate that acclimation to moderate salt stress modifies stress perception and the activation of several downstream pathways. [Display omitted] •Osmotic stress triggers several phospholipid signalling pathways in Chlamydomonas.•Salt-acclimation affects both growth and phospholipid-signalling responses.•Lipid responses in acclimated cells are lower, and shifted to higher osmotic stress levels.•Lipid responses involve five independent routes, i.e. PI(3,5)P2, PI(4,5)P2, PA, DGPP and LPA.•Acclimation to moderate salt stress modifies stress perception and downstream signalling pathways.
ISSN:0031-9422
1873-3700
DOI:10.1016/j.phytochem.2016.12.014