Identification of a cyclodextrin inclusion complex of antimicrobial peptide CM4 and its antimicrobial activity

•β-CD was chosen to form an inclusion complex with ABP-CM4.•The physical and chemical properties of β-CD/CM4 complex were tested.•The β-CD/CM4 complex exhibited good antimicrobial activity in vitro and in vivo.•The β-CD/CM4 complex could be suitable for use in the food industry as an antimicrobial s...

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Bibliographic Details
Published in:Food chemistry 2017-04, Vol.221, p.296-301
Main Authors: Li, Jian-Feng, Zhang, Jia-Xin, Wang, Zhi-Guo, Yao, Ying-Jia, Han, Xu, Zhao, You-Long, Liu, Jun-Ping, Zhang, Shuang-Quan
Format: Article
Language:English
Subjects:
ABP-CM4
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antibacterial assay
Antimicrobial Cationic Peptides - chemistry
Antimicrobial Cationic Peptides - pharmacology
Bacteria - drug effects
beta-Cyclodextrins - chemistry
Fruit - drug effects
Fruit - microbiology
Inclusion complex
Stability
β-Cyclodextrin
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Summary:•β-CD was chosen to form an inclusion complex with ABP-CM4.•The physical and chemical properties of β-CD/CM4 complex were tested.•The β-CD/CM4 complex exhibited good antimicrobial activity in vitro and in vivo.•The β-CD/CM4 complex could be suitable for use in the food industry as an antimicrobial system. Antibacterial peptide CM4 (ABP-CM4) is a natural product isolated from the silkworm Bombyx mori. It is a small cationic peptide with broad-spectrum activities against harmful microorganisms and may be used as a novel food preservative. However, ABP-CM4 lacks tertiary structure in water-like solutions, which makes it more susceptible to proteases and labile when exposed to air. In this study, β-cyclodextrin (β-CD) was chosen to form an inclusion complex with ABP-CM4, which enhanced the physical and chemical properties of ABP-CM4 but did not decrease its antibacterial activity. The storage stability and susceptibility to proteinases of ABP-CM4 were apparently improved under the protection of β-CD. This technology could also be widely applied to other AMPs as an antimicrobial system to be used in the food industry.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2016.10.040