Listeria monocytogenes induces mast cell extracellular traps

Abstract Mast cells play an essential role in different immunological phenomena including allergy and infectious diseases. Several bacteria induce mast cell activation leading to degranulation and the production of several cytokines and chemokines. However, mast cells also have different microbicida...

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Bibliographic Details
Published in:Immunobiology (1979) 2017-02, Vol.222 (2), p.432-439
Main Authors: Campillo-Navarro, Marcia, Leyva-Paredes, Kahiry, Donis-Maturano, Luis, González-Jiménez, Marco, Paredes-Vivas, Yuriria, Cerbulo-Vázquez, Arturo, Serafín-López, Jeanet, García-Pérez, Blanca, Ullrich, Stephen E, Flores-Romo, Leopoldo, Pérez-Tapia, Sonia M, Estrada-Parra, Sergio, Estrada-García, Iris, Chacón-Salinas, Rommel
Format: Article
Language:English
Subjects:
Advanced Basic Science
Allergy and Immunology
beta-N-Acetylhexosaminidases - metabolism
Cell Line
DNA - metabolism
Extracellular Traps - immunology
Extracellular Traps - metabolism
Histones - metabolism
Host-Pathogen Interactions - immunology
Humans
Listeria monocytogenes
Listeria monocytogenes - immunology
Listeriosis
Mast cell
Mast cell extracellular trap
Mast Cells - immunology
Mast Cells - metabolism
Mast Cells - ultrastructure
Nuclear Envelope - ultrastructure
Phagocytosis - immunology
Reactive Oxygen Species - metabolism
β-Hexosaminidase
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Summary:Abstract Mast cells play an essential role in different immunological phenomena including allergy and infectious diseases. Several bacteria induce mast cell activation leading to degranulation and the production of several cytokines and chemokines. However, mast cells also have different microbicidal activities such as phagocytosis and the release of DNA with embedded granular proteins known as Mast Cell Extracellular Traps (MCETs). Although previous reports indicate that extracellular bacteria are able to induce MCETs little is known if intracellular bacteria can induce these structures. In this work, we evaluated MCETs induction by the intracellular bacteria Listeria monocytogenes . We found that mast cells released DNA after stimulation with L. monocytogenes , and this DNA was complexed to histone and tryptase. Before extracellular DNA release, L. monocytogenes induced modifications to the mast cell nuclear envelope and DNA was detected outside the nucleus. L. monocytogenes stimulated mast cells to produce significant amounts of reactive oxygen species (ROS) and blocking NADPH oxidase diminished DNA release by mast cells. Finally, MCETs showed antimicrobial activity against L. monocytogenes that was partially blocked when β-hexosaminidase activity was inhibited. These results show that L. monocytogenes induces mast cells to produce microbicidal MCETs, suggesting a role for mast cells in containing infection beyond the induction of inflammation.
ISSN:0171-2985
1878-3279
DOI:10.1016/j.imbio.2016.08.006