Subretinal Fluid Levels of Signal-Transduction Proteins and Apoptosis Molecules in Macula-Off Retinal Detachment Undergoing Scleral Buckle Surgery

To evaluate signal transduction and early apoptosis protein levels in subretinal fluid collected during scleral buckling surgery for macula-off rhegmatogenous retinal detachment (RRD). Our aim was to assess both their relation with RRD features and their influence on the posttreatment outcome. Thirt...

Full description

Saved in:
Bibliographic Details
Published in:Investigative ophthalmology & visual science 2016-12, Vol.57 (15), p.6895-6901
Main Authors: Carpineto, Paolo, Aharrh-Gnama, Agbeanda, Ciciarelli, Vincenzo, Borrelli, Enrico, Petti, Francesco, Aloia, Raffaella, Lamolinara, Alessia, Di Nicola, Marta, Mastropasqua, Leonardo
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Apoptosis
Caspase 8 - metabolism
Caspase 9 - metabolism
Female
Humans
Macula Lutea - metabolism
Macula Lutea - pathology
Male
Middle Aged
Retinal Detachment - metabolism
Retinal Detachment - pathology
Retinal Detachment - surgery
Retrospective Studies
Scleral Buckling - methods
Signal Transduction
Subretinal Fluid - metabolism
Tomography, Optical Coherence - methods
Visual Acuity
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To evaluate signal transduction and early apoptosis protein levels in subretinal fluid collected during scleral buckling surgery for macula-off rhegmatogenous retinal detachment (RRD). Our aim was to assess both their relation with RRD features and their influence on the posttreatment outcome. Thirty-three eyes of 33 RRD patients scheduled for scleral buckle surgery were enrolled in the study. Undiluted subretinal fluid samples were collected during surgery and analyzed via magnetic bead-based immunoassay. All patients underwent a complete ophthalmologic evaluation at baseline and at each follow-up visit (months 1, 3, and 6). Moreover, both at baseline and at the postsurgery month 6 visit, the patients were tested by means of spectral-domain optical coherence tomography (SD-OCT) in order to evaluate the average ganglion cell-inner plexiform complex thickness, as well as the photoreceptor inner segment/outer segment junction status. Patients' clinical features (retinal detachment size, detachment duration, and occurrence of proliferative vitreoretinopathy) were associated with several early apoptotic factors (caspase-8, caspase-9, and B-cell lymphoma 2 [Bcl-2]-associated death promoter [BAD]). Furthermore, both early apoptosis factors (caspase-8, Bcl-2, and p53) and signal-transduction proteins (ERK 1/2) were found to influence the postsurgery month 3 OCT characteristics. Signal-transduction proteins and early apoptosis proteins are associated with different clinical features and postsurgery outcomes.
ISSN:1552-5783
1552-5783
DOI:10.1167/iovs.16-20372