Characterization of putative virulence factors of Serratia marcescens strain SEN for pathogenesis in Spodoptera litura

[Display omitted] •Virulence factors of entomopathogenic Serratia marcescens were characterized.•The virulent S. marcescens strain SEN possessed higher hydrolytic enzymes activity.•It showed higher relative expression of the three toxin genes shlA, phlA and swr.•It was able to proliferate considerab...

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Bibliographic Details
Published in:Journal of invertebrate pathology 2017-02, Vol.143, p.115-123
Main Authors: Aggarwal, Chetana, Paul, Sangeeta, Tripathi, Vishwas, Paul, Bishwajeet, Khan, Md. Aslam
Format: Article
Language:English
Subjects:
Animals
Chitinase
Gene Expression Profiling
Hemolysin
Peritrophic membrane
Polymerase Chain Reaction
Protease
Serratia Infections - veterinary
Serratia marcescens
Serratia marcescens - pathogenicity
Spodoptera - microbiology
Spodoptera litura
Transcriptome
Virulence - physiology
Virulence Factors - biosynthesis
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Summary:[Display omitted] •Virulence factors of entomopathogenic Serratia marcescens were characterized.•The virulent S. marcescens strain SEN possessed higher hydrolytic enzymes activity.•It showed higher relative expression of the three toxin genes shlA, phlA and swr.•It was able to proliferate considerably in Spodoptera litura gut and hemolymph.•Peritrophic membrane of the treated larvae treated showed considerable damage. Two Serratia marcescens strains, SEN and ICC-4, isolated from diseased insect cadavers were observed to differ considerably in their virulence towards Spodoptera litura. The present study was aimed to characterize the possible virulence factors present in the virulent Serratia marcescens strain SEN. Both the S. marcescens strains were evaluated for the presence of various lytic enzymes such as chitinase, lipase, protease and phospholipase. The virulent S. marcescens strain SEN was observed to possess considerably higher activity of chitinase and protease enzymes; activity of phospholipase enzyme was also higher. Although, all the three toxin genes shlA, phlA and swr could be detected in both the S. marcescens strains, there was a higher expression of these genes in the virulent strain SEN. S. marcescens strain ICC-4 showed greater reduction in overall growth yield in the post-exponential phase in the presence of midgut juice and hemolymph of S. litura larvae, as compared to S. marcescens strain SEN. Proliferation of the S. marcescens strain SEN was also considerably higher in foregut, midgut and hemolymph of S. litura larvae, as compared to strain ICC-4. Peritrophic membrane treated with broth culture of the S. marcescens strain SEN showed higher damage as compared to strain ICC-4. The peritrophic membrane of larvae fed on diet treated with the virulent strain showed considerable damage while the peritrophic membrane of larvae fed on diet treated with the non-virulent strain showed no damage. This is the first report documenting the fate of ingested S. marcescens in S. litura gut and the relative expression of toxin genes from two S. marcescens strains differing in their virulence towards S. litura.
ISSN:0022-2011
1096-0805
DOI:10.1016/j.jip.2016.12.004