An optimized high quality male DNA extraction from spermatophores in open thelycum shrimp species

The crucial step of most of the current genetic studies is the extraction of DNA of sufficient quantity and quality. Several genomic DNA isolation methods have been described to successfully obtain male DNA from shrimp species. However, all current protocols require invasive handling methods with ma...

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Bibliographic Details
Published in:Integrative zoology 2017-09, Vol.12 (5), p.421-427
Main Authors: PLANELLA, Laia, HERAS, Sandra, VERA, Manuel, GARCÍA‐MARÍN, José‐Luis, ROLDÁN, María Inés
Format: Article
Language:English
Subjects:
Animals
Aquaculture
Deoxyribonucleic acid
differential spermatophore DNA extraction
DNA
DNA - analysis
Female
Genotype
Genotyping
Handling
high quality DNA
Insects
Male
male genotyping
Males
Mating
Methods
non‐invasive DNA extraction
Nucleotide sequence
open thelycum shrimp
PCR
Penaeidae - genetics
Polymerase Chain Reaction
Reproduction
Reproductive behaviour
Selective breeding
Shellfish
Shrimp culture
Species
Spermatogonia
Spermatophores
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Summary:The crucial step of most of the current genetic studies is the extraction of DNA of sufficient quantity and quality. Several genomic DNA isolation methods have been described to successfully obtain male DNA from shrimp species. However, all current protocols require invasive handling methods with males for DNA isolation. Using Aristeus antennatus as a model we tested a reliable non‐invasive differential DNA extraction method to male DNA isolation from spermatophores attached to female thelycum. The present protocol provides high quality and quantity DNA for polymerase chain reaction amplification and male genotyping. This new approach could be useful to experimental shrimp culture to select sires with relevant genetic patterns for selective breeding programs. More importantly, it can be applied to identify the mating pairs and male structure in wild populations of species as A. antennatus, where males are often difficult to capture. Our method could be also valuable for biological studies on other spermatophore‐using species, such as myriapods, arachnids and insects.
ISSN:1749-4877
1749-4869
1749-4877
DOI:10.1111/1749-4877.12250