DNase concentration assay to obtain DNA-free RNA from sugarcane leaves

The success of gene expression studies, protein synthesis, and construction of cDNA libraries directly depends on the purity and integrity of the RNA used in these tests, as even minor amounts of contaminant DNA (

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Bibliographic Details
Published in:Genetics and molecular research 2016-12, Vol.15 (4)
Main Authors: Santos, J A, Luz, G A, Oliveira, K P, Oliveira, L F, Andrade Júnior, A S, Valente, S E S, Lima, P S C
Format: Article
Language:English
Subjects:
Deoxyribonucleases - metabolism
DNA - metabolism
Gene Expression
Plant Leaves - genetics
RNA, Plant - analysis
RNA, Plant - standards
Saccharum - genetics
Online Access:Get full text
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Description
Summary:The success of gene expression studies, protein synthesis, and construction of cDNA libraries directly depends on the purity and integrity of the RNA used in these tests, as even minor amounts of contaminant DNA (
ISSN:1676-5680
1676-5680
DOI:10.4238/GMR15049073