The role of amides in seminal cryopreservation of wild silverside, Odontesthes bonariensis

Amides were tested as internal cryoprotectants for the preservation of wild silverside (Odontesthes bonariensis) sperm. The semen was diluted in modified Mounib's medium and cryopreserved by adding 2, 5, 8 or 11% of dimethyl acetamide (DMA), dimethyl formamide (DMF) or methyl formamide (MF). Di...

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Bibliographic Details
Published in:Cryobiology 2016-12, Vol.73 (3), p.383-387
Main Authors: Alves, Juliana P., Corcini, Carine D., Silva, Estela F., Caldas, Josie S., Cardoso, Tainã F., Piedras, Sergio R.N., Jardim, Rodrigo D., Varela, Antonio S.
Format: Article
Language:English
Subjects:
Acetamides - pharmacology
Animals
Atheriniformes
Cell Membrane - drug effects
Conservation
Cryopreservation - methods
Cryoprotective Agents - pharmacology
Dimethyl Sulfoxide - pharmacology
Dimethylformamide - pharmacology
Fishes
Formamides - pharmacology
Freezing
Male
Odontesthes bonariensis
Semen - drug effects
Semen Preservation - methods
Sperm Motility - drug effects
Spermatozoa
Spermatozoa - drug effects
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Summary:Amides were tested as internal cryoprotectants for the preservation of wild silverside (Odontesthes bonariensis) sperm. The semen was diluted in modified Mounib's medium and cryopreserved by adding 2, 5, 8 or 11% of dimethyl acetamide (DMA), dimethyl formamide (DMF) or methyl formamide (MF). Dimethyl sulfoxide (DMSO) at a concentration of 10% diluted in modified Mounib's medium was used as a control. The rate motility (17.7 ± 1.9%) and time motility (143.2 ± 9.7 s) (P 
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2016.09.001