Biodynamics of copper oxide nanoparticles and copper ions in an oligochaete - Part II: Subcellular distribution following sediment exposure

•L. variegatus was exposed to sediment spiked with either aqueous Cu or nanoparticulate CuO.•Both aqueous and nanoparticulate Cu were marginally accumulated by L. variegatus.•Elimination of Cu accumulated from both forms was limited.•The subcellular distribution of accumulated Cu varied between Cu f...

Full description

Saved in:
Bibliographic Details
Published in:Aquatic toxicology 2016-11, Vol.180, p.25-35
Main Authors: Thit, Amalie, Ramskov, Tina, Croteau, Marie-Noële, Selck, Henriette
Format: Article
Language:English
Subjects:
Animals
Biological Availability
Body Burden
Copper - pharmacokinetics
Fresh Water
Freshwater
Geologic Sediments - chemistry
Ions - pharmacokinetics
Lumbriculus variegatus
Metal
Metal Nanoparticles
Oligochaeta
Oligochaeta - metabolism
Sediment
Stable isotope tracer
Subcellular Fractions - chemistry
Water Pollutants, Chemical - pharmacokinetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•L. variegatus was exposed to sediment spiked with either aqueous Cu or nanoparticulate CuO.•Both aqueous and nanoparticulate Cu were marginally accumulated by L. variegatus.•Elimination of Cu accumulated from both forms was limited.•The subcellular distribution of accumulated Cu varied between Cu forms.•The use of a tracer, greater exposure concentration and duration are recommended. The use and likely incidental release of metal nanoparticles (NPs) is steadily increasing. Despite the increasing amount of published literature on metal NP toxicity in the aquatic environment, very little is known about the biological fate of NPs after sediment exposures. Here, we compare the bioavailability and subcellular distribution of copper oxide (CuO) NPs and aqueous Cu (Cu-Aq) in the sediment-dwelling worm Lumbriculus variegatus. Ten days (d) sediment exposure resulted in marginal Cu bioaccumulation in L. variegatus for both forms of Cu. Bioaccumulation was detected because isotopically enriched 65Cu was used as a tracer. Neither burrowing behavior or survival was affected by the exposure. Once incorporated into tissue, Cu loss was negligible over 10 d of elimination in clean sediment (Cu elimination rate constants were not different from zero). With the exception of day 10, differences in bioaccumulation and subcellular distribution between Cu forms were either not detectable or marginal. After 10 d of exposure to Cu-Aq, the accumulated Cu was primarily partitioned in the subcellular fraction containing metallothionein-like proteins (MTLP, ≈40%) and cellular debris (CD, ≈30%). Cu concentrations in these fractions were significantly higher than in controls. For worms exposed to CuO NPs for 10 d, most of the accumulated Cu was partitioned in the CD fraction (≈40%), which was the only subcellular fraction where the Cu concentration was significantly higher than for the control group. Our results indicate that L. variegatus handle the two Cu forms differently. However, longer-term exposures are suggested in order to clearly highlight differences in the subcellular distribution of these two Cu forms.
ISSN:0166-445X
1879-1514
DOI:10.1016/j.aquatox.2016.08.011