Detection and quantification of HIV-1 RNA with a fully automated transcription-mediated-amplification assay

Highlights • The Limit of detection of the TMA-based assay was 8.6 [CI95%:6.7–13.7] c/ml. • Using reference panels, APTIMA was accurate, precise, reproducible and sensitive. • Using clinical panel, APTIMA and CAPCTM vs 2.0 assays agreed perfectly.

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Bibliographic Details
Published in:Journal of clinical virology 2016-11, Vol.84, p.70-73
Main Authors: Sauné, K, Raymond, S, Boineau, J, Pasquier, C, Izopet, J
Format: Article
Language:English
Subjects:
Allergy and Immunology
Aptima HIV Quant Dx assay
Automation, Laboratory
HIV Infections - diagnosis
HIV Infections - virology
HIV RNA quantification
HIV-1 - genetics
HIV-1 - isolation & purification
Humans
Infectious Disease
Lentivirus
Limit of Detection
Lower limit of detection
Nucleic Acid Amplification Techniques - instrumentation
Nucleic Acid Amplification Techniques - methods
Reagent Kits, Diagnostic
Real-Time Polymerase Chain Reaction - instrumentation
Real-Time Polymerase Chain Reaction - methods
Reproducibility of Results
Retroviridae
RNA, Viral - blood
RNA, Viral - genetics
RNA, Viral - isolation & purification
Roche CAP/CTM HIV-1 version 2.0
Sensitivity and Specificity
Viral Load
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Description
Summary:Highlights • The Limit of detection of the TMA-based assay was 8.6 [CI95%:6.7–13.7] c/ml. • Using reference panels, APTIMA was accurate, precise, reproducible and sensitive. • Using clinical panel, APTIMA and CAPCTM vs 2.0 assays agreed perfectly.
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2016.09.002