Frequent Alterations of the β-Catenin Protein in Cancer of the Uterine Cervix

Cancer of the uterine cervix is still the leading cause of death among women with cancer in developing countries. Although infections with human papillomavirus are necessary, other molecular alterations that are needed at the cellular level for development of these tumors remain largely unknown. β-C...

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Bibliographic Details
Published in:Tumor biology 2002-01, Vol.23 (1), p.45-53
Main Authors: Pereira-Suárez, Ana Laura, Meraz, Marco Antonio, Lizano, Marcela, Estrada-Chávez, Ciro, Hernández, Fernando, Olivera, Primitivo, Pérez, Elizabeth, Padilla, Patricia, Yaniv, Moshe, Thierry, Françoise, García-Carrancá, Alejandro
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Base Sequence
beta Catenin
Biological and medical sciences
Blotting, Western
Cell Nucleus - metabolism
Cytoplasm - metabolism
Cytoskeletal Proteins - biosynthesis
Cytoskeletal Proteins - genetics
Exons
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Humans
Immunoblotting
Immunohistochemistry
Medical sciences
Middle Aged
Molecular Sequence Data
Mutation
Polymorphism, Single-Stranded Conformational
Proto-Oncogene Proteins - metabolism
Research Article
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Trans-Activators
Tumor Cells, Cultured
Tumors
Uterine Neoplasms - genetics
Uterine Neoplasms - metabolism
Wnt Proteins
Zebrafish Proteins
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Summary:Cancer of the uterine cervix is still the leading cause of death among women with cancer in developing countries. Although infections with human papillomavirus are necessary, other molecular alterations that are needed at the cellular level for development of these tumors remain largely unknown. β-Catenin is a key regulator located within the Wnt signaling cascade whose alterations constitute an important event in colon carcinogenesis. In many malignancies increased levels of the β-catenin protein have been found, associated with its nuclear and/or cytoplasmic accumulation. To search for possible alterations of this pathway we examined the expression and localization of the β-catenin protein in tumors from the uterine cervix and cell lines derived from them. β-Catenin was found accumulated in the cytoplasm and/or nuclei of 12 out of 32 samples. In accordance, increased levels of this protein were observed in 9 out of 20 tumors analyzed. Importantly, PCR-SSCP and sequence analysis showed no mutations in exons 3, 4 and 6 of the β-catenin gene. Our findings indicate that alterations of β-catenin are frequent in these tumors and suggest that they may play an important role in the development of cancer of the uterine cervix. They also indicate that higher protein levels and abnormal localization may result from several different mechanisms.
ISSN:1010-4283
1423-0380
DOI:10.1159/000048688