Molecular characterization and immunological response analysis of toll-like receptors from the blunt snout bream (Megalobrama amblycephala)

The innate immunity serves as the primary line of defense against pathogen invasion. Toll-like receptors (TLRs) play a pivotal role in the innate immunity by sensing specific pathogen-associated molecular patterns and activating immune responses. In this study, 14 TLRs (TLR1, 2, 3, 4, 5, 7, 8a, 8b,...

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Published in:	Developmental and comparative immunology 2017-02, Vol.67, p.471-475
Main Authors:	Lai, Rui-Fang, Jakovlić, Ivan, Liu, Han, Zhan, Fan-Bin, Wei, Jin, Wang, Wei-Min
Format:	Article
Language:	eng
Subjects:	Aeromonas hydrophila Aeromonas hydrophila - immunology Animal tissues Animals Bacterial infections Bream Cloning, Molecular Cyprinidae - immunology Expression Fish Fish Diseases - immunology Fish Proteins - genetics Fish Proteins - metabolism Gene expression Gram-Negative Bacterial Infections - immunology Head Kidney - metabolism Immune response Immune system Immunity Immunity, Innate - genetics Immunology Innate immunity Kidneys Liver Liver - metabolism Megalobrama amblycephala Pathogen-Associated Molecular Pattern Molecules - immunology Pathogens Phylogeny Protein Domains - genetics Proteins Receptors Spleen TLR TLR1 protein Toll-like receptors Toll-Like Receptors - genetics Toll-Like Receptors - metabolism Transcriptome
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Summary:	The innate immunity serves as the primary line of defense against pathogen invasion. Toll-like receptors (TLRs) play a pivotal role in the innate immunity by sensing specific pathogen-associated molecular patterns and activating immune responses. In this study, 14 TLRs (TLR1, 2, 3, 4, 5, 7, 8a, 8b, 9, 18, 19, 20, 21 and 22) were identified and characterized in a cyprinid fish Megalobrama amblycephala. A majority of MaTLRs possessed the typical structural features of the TLR protein family: LRR domain, TM region and TIR domain. Phylogenetic analyses confirmed the existence of six TLR families and revealed close relationships with other cyprinid orthologs. All TLRs were constitutively expressed in all eight examined tissues. After Aeromonas hydrophila challenge, the expression profiles of MaTLR mRNAs were analyzed in liver, spleen and head kidney: MaTLR1, 2, 5, 9, 21 and 22 were up-regulated, MaTLR3, 7, 8a, 8b and 19 were down-regulated, and MaTLR4, 18 and 20 expression patterns varied among tissues. These results indicate that most of MaTLRs are likely to be involved in the immune responses against bacterial infection. •14 TLR genes were characterized and analyzed in Megalobrama amblycephala.•Phylogenetic tree of MaTLRs obviously showed the existence of six TLR families.•All MaTLRs were constitutively expressed with variations in eight tested tissues.•The expression profiles of MaTLRs were analyzed after A. hydrophila infection.•Results indicated that most of MaTLRs might involve in the immune responses.
ISSN:	0145-305X 1879-0089