Tissue distribution of ochratoxin A as determined by HPLC and ELISA and histopathological effects in chickens

Ochratoxin A is a common feed contaminant, which may impair animal health and may lead to residues in edible tissues of slaughter animals. To simulate field conditions, broiler chicks were exposed to a total of 0.5 mg ochratoxin A per week for each of 4 weeks. Plasma toxin levels and tissue residues...

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Bibliographic Details
Published in:Avian pathology 2002-04, Vol.31 (2), p.141-148
Main Authors: Biró, Krisztina, Solti, László, Barna-Vetró, Ildikó, Bagó, György, Glávits, Róbert, Szabó, Erzsébet, Fink-Gremmels, Johanna
Format: Article
Language:English
Subjects:
Animals
Birds
Body Weight - drug effects
Chickens
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Food Contamination
Kidney - drug effects
Kidney - pathology
Ochratoxins - pharmacokinetics
Ochratoxins - toxicity
Pathology
Poultry
Tissue Distribution
Urothelium - drug effects
Urothelium - pathology
Urothelium - ultrastructure
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Summary:Ochratoxin A is a common feed contaminant, which may impair animal health and may lead to residues in edible tissues of slaughter animals. To simulate field conditions, broiler chicks were exposed to a total of 0.5 mg ochratoxin A per week for each of 4 weeks. Plasma toxin levels and tissue residues were measured by high-performance liquid chromatography (HPLC) and microplate enzyme-linked immunosorbent assay (ELISA). Results indicate an accumulation in plasma and wide distribution into all organs, with high levels in the liver and the kidney. Microscopical changes that could primarily be associated with toxin exposure were glomerulonephrosis, tubulonephrosis, focal tubular epithelial cell proliferation and multiple, adenoma-like structures in the renal parenchyma. The HPLC and ELISA methods gave similar results for both tissue distribution and depletion. Differences in absolute tissue toxin concentrations obtained by the two methods might be attributed to the different extraction and clean-up procedures, along with antibody specificity. The findings indicate that the dose applied causes subclinical tissue lesions and measurable tissue residues.
ISSN:0307-9457
1465-3338
DOI:10.1080/03079450120118621