Essential role of K+ uptake permease (Kup) for resistance to sucrose‐induced stress in Gluconacetobacter diazotrophicus PAl 5

Summary Microorganisms are constantly challenged by stressful conditions, such as sugar‐rich environments. Such environments can cause an imbalance of biochemical activities and compromise cell multiplication. Gluconacetobacter diazotrophicus PAl 5 is among the most sugar‐tolerant bacteria, capable...

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Bibliographic Details
Published in:Environmental microbiology reports 2017-04, Vol.9 (2), p.85-90
Main Authors: de Oliveira, Marcos V. V., Intorne, Aline C., Vespoli, Luciano de S., Andrade, Leandro F., Pereira, Leandro de M., Rangel, Patrícia L., de Souza Filho, Gonçalo A.
Format: Article
Language:English
Subjects:
Affinity
Amino acids
Bacteria
Complementation
DNA Transposable Elements
Genes
Genetic Complementation Test
Genomes
Gluconacetobacter - drug effects
Gluconacetobacter - genetics
Gluconacetobacter - physiology
Gluconacetobacter diazotrophicus
Membrane Transport Proteins - metabolism
Microorganisms
Molecular modelling
Mutagenesis, Insertional
Mutants
Osmotic Pressure
Permease
Phenotypes
Plant growth
Potassium
Potassium - metabolism
Potassium transporter
Protein transport
Stress, Physiological
Sucrose
Sucrose - metabolism
Sugar
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Summary:Summary Microorganisms are constantly challenged by stressful conditions, such as sugar‐rich environments. Such environments can cause an imbalance of biochemical activities and compromise cell multiplication. Gluconacetobacter diazotrophicus PAl 5 is among the most sugar‐tolerant bacteria, capable of growing in the presence of up to 876 mM sucrose. However, the molecular mechanisms involved in its response to high sucrose remain unknown. The present work aimed to identify sucrose‐induced stress resistance genes in G. diazotrophicus PAl 5. Screening of a Tn5 transposon insertion library identified a mutant that was severely compromised in its resistance to high sucrose concentrations. Molecular characterization revealed that the mutation affected the kupA gene, which encodes a K+ uptake transporter (KupA). Functional complementation of the mutant with the wild type kupA gene recovered the sucrose‐induced stress resistance phenotype. High sucrose resistance assay, under different potassium concentrations, revealed that KupA acts as a high‐affinity K+ transporter, which is essential for resistance to sucrose‐induced stress, when extracellular potassium levels are low. This study is the first to show the essential role of the KupA protein for resistance to sucrose‐induced stress in bacteria by acting as a high‐affinity potassium transporter in G. diazotrophicus PAl 5.
ISSN:1758-2229
1758-2229
DOI:10.1111/1758-2229.12503