Dek35 Encodes a PPR Protein that Affects cis-Splicing of Mitochondrial nad4 Intron 1 and Seed Development in Maize

In higher plants, the splicing of organelle-encoded mRNA involves a complex collaboration with nuclear- encoded proteins. Pentatricopeptide repeat （PPR） proteins have been implicated in these RNA-protein interactions. In this study, we performed the cloning and functional characterization of maize D...

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Bibliographic Details
Published in:Molecular plant 2017-03, Vol.10 (3), p.427-441
Main Authors: Chen, Xinze, Feng, Fan, Qi, Weiwei, Xu, Liming, Yao, Dongsheng, Wang, Qun, Song, Rentao
Format: Article
Language:English
Subjects:
CIS
Gene Expression Regulation, Plant - genetics
Introns - genetics
maize
mitochondria
Mitochondria - genetics
Mitochondria - metabolism
Mitochondrial Proteins - genetics
Mitochondrial Proteins - metabolism
NAD - metabolism
NADH
Plant Proteins - genetics
Plant Proteins - metabolism
PPR
PR蛋白
RNA Splicing - genetics
RNA Splicing - physiology
Seeds - genetics
Seeds - growth & development
Seeds - metabolism
splicing
Zea mays - genetics
Zea mays - growth & development
Zea mays - metabolism
内含子
玉米种子
突变等位基因
线粒体
编码蛋白
蛋白质相互作用
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Summary:In higher plants, the splicing of organelle-encoded mRNA involves a complex collaboration with nuclear- encoded proteins. Pentatricopeptide repeat （PPR） proteins have been implicated in these RNA-protein interactions. In this study, we performed the cloning and functional characterization of maize Defective kernel 35 （Dek35）. The dek35-ref mutant is a lethal-seed mutant with developmental deficiency. Dek35 was cloned through Mutator tag isolation and further confirmed by four additional independent mutant alleles. Dek35 encodes an P-type PPR protein that targets the mitochondria. The dek35 mutation causes significant reduction in the accumulation of DEK35 proteins and reduced splicing efficiency of mitochon- drial nad4 intron 1. Analysis of mitochondrial complex in dek35 immature seeds indicated severe deficiency in the complex I assembly and NADH dehydrogenase activity. Transcriptome analysis of dek35 endosperm revealed enhanced expression of genes involved in the alternative respiratory pathway and extensive differentially expressed genes related to mitochondrial function and activity. Collectively, these results indicate that Dek35 encodes an PPR protein that affects the cis-splicing of mitochondrial nad4 intron 1 and is required for mitochondrial function and seed development.
ISSN:1674-2052
1752-9867
DOI:10.1016/j.molp.2016.08.008