Quantification of fluorescence angiography in a porcine model

Purpose There is no consensus on how to quantify indocyanine green (ICG) fluorescence angiography. The aim of the present study was to establish and gather validity evidence for a method of quantifying fluorescence angiography, to assess organ perfusion. Methods Laparotomy was performed on seven pig...

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Bibliographic Details
Published in:Langenbeck's archives of surgery 2017-06, Vol.402 (4), p.655-662
Main Authors: Nerup, Nikolaj, Andersen, Helene Schou, Ambrus, Rikard, Strandby, Rune Broni, Svendsen, Morten Bo Søndergaard, Madsen, Mads Holst, Svendsen, Lars Bo, Achiam, Michael Patrick
Format: Article
Language:English
Subjects:
Abdominal Surgery
Algorithms
Animals
Cardiac Surgery
Coloring Agents
Digestive System - blood supply
Digestive System - diagnostic imaging
Fluorescein Angiography
General Surgery
Indocyanine Green
Laparotomy
Medicine
Medicine & Public Health
Models, Animal
Original Article
Regional Blood Flow - physiology
Reproducibility of Results
Swine
Thoracic Surgery
Traumatic Surgery
Vascular Surgery
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Summary:Purpose There is no consensus on how to quantify indocyanine green (ICG) fluorescence angiography. The aim of the present study was to establish and gather validity evidence for a method of quantifying fluorescence angiography, to assess organ perfusion. Methods Laparotomy was performed on seven pigs, with two regions of interest (ROIs) marked. ICG and neutron-activated microspheres were administered and the stomach was illuminated in the near-infrared range, parallel to continuous recording of fluorescence signal. Tissue samples from the ROIs were sent for quantification of microspheres to calculate the regional blood flow. A software system was developed to assess the fluorescent recordings quantitatively, and each quantitative parameter was compared with the regional blood flow. The parameter with the strongest correlation was then compared with results from an independently developed algorithm, to evaluate reproducibility. Results A strong correlation was found between regional blood flow and the slope of the fluorescence curves (ROI I: Pearson r  = 0.97, p  
ISSN:1435-2443
1435-2451
DOI:10.1007/s00423-016-1531-z