Twig pre-harvest temperature significantly influences effective cryopreservation of Vaccinium dormant buds

Cryopreservation of temperate woody-plant material by dormant buds is less expensive than using shoot tips isolated from tissue cultured plants; however currently, dormant buds are used only for preservation of selected temperate tree and shrub species. Using dormant buds could be an efficient strat...

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Bibliographic Details
Published in:Cryobiology 2017-02, Vol.74, p.154-159
Main Authors: Jenderek, Maria M., Tanner, Justin D., Ambruzs, Barbara D., West, Mark, Postman, Joseph D., Hummer, Kim E.
Format: Article
Language:English
Subjects:
Blueberry
Cryopreservation - methods
Desiccation
Gene banking
Plant Shoots - growth & development
Plant Stems - growth & development
Post cryopreservation regrowth
Regression models
Seasons
Temperature
Vaccinium - growth & development
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Summary:Cryopreservation of temperate woody-plant material by dormant buds is less expensive than using shoot tips isolated from tissue cultured plants; however currently, dormant buds are used only for preservation of selected temperate tree and shrub species. Using dormant buds could be an efficient strategy for long-term preservation of blueberry (Vaccinium L.) genetic resources. In this study, viability of V. hybrid ‘Northsky’ (PI 554943) dormant buds was evaluated at 30 harvest dates over three consecutive fall/winter seasons to determine the optimal harvest time that promotes high post cryopreservation viability. Twigs with dormant buds were cut into 70 mm segments containing at least two nodes, desiccated, slowly cooled, stored in liquid nitrogen vapor and tested for post-cryopreservation regrowth. The highest regrowth of cryopreserved dormant buds was observed for buds harvested in mid-December and during the first half of January. Pearson's correlation coefficients were computed to evaluate the association between bud characteristics and viability at harvest date and logistic regression models were fit to test the ability of twig characteristics and temperatures to predict post cryopreservation bud viability. Post-cryopreservation viability was negatively correlated (p 
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2016.10.007