Effects of sleeve gastrectomy and rs9930506 FTO variants on angiopoietin/Tie-2 system in fat expansion and M1 macrophages recruitment in morbidly obese subjects

Angiogenesis in inflammation are hallmarks for adipose tissue expansion in obesity. The role of angiopoietin/Tie-2 system in adipose tissue expansion and immune cell recruitment is unclear. We studied the effect of sleeve gastrectomy and the influence of FTO rs9930506 polymorphism on Tie-2, angiopoi...

Full description

Saved in:
Bibliographic Details
Published in:Endocrine 2016-12, Vol.54 (3), p.700-713
Main Authors: Figueroa-Vega, Nicté, Jordán, Benjamín, Pérez-Luque, Elva Leticia, Parra-Laporte, Luis, Garnelo, Serafín, Malacara, Juan Manuel
Format: Article
Language:English
Subjects:
Adult
Alpha-Ketoglutarate-Dependent Dioxygenase FTO - genetics
Angiopoietins - metabolism
Anthropometry
Diabetes
Endocrinology
Female
Gastrectomy
Humanities and Social Sciences
Humans
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Internal Medicine
Macrophages
Macrophages - metabolism
Male
Medicine
Medicine & Public Health
Middle Aged
multidisciplinary
Obesity, Morbid - immunology
Obesity, Morbid - metabolism
Obesity, Morbid - surgery
Original Article
Polymorphism, Single Nucleotide
Receptor, TIE-2 - metabolism
Science
Subcutaneous Fat - immunology
Subcutaneous Fat - metabolism
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Angiogenesis in inflammation are hallmarks for adipose tissue expansion in obesity. The role of angiopoietin/Tie-2 system in adipose tissue expansion and immune cell recruitment is unclear. We studied the effect of sleeve gastrectomy and the influence of FTO rs9930506 polymorphism on Tie-2, angiopoietin-1 and angiopoietin-2 expression in morbid obesity. Fifteen morbidly obese subjects (4 men and 11 women) aged 24–55 years were followed-up 3 and 6 months after sleeve gastrectomy. Serum sTie-2, angiopoietin-1, angiopoietin-2, and hypoxia-inducible factor-1α concentrations were determined by ELISA. Tie-2 and its ligands in visceral and subcutaneous adipose tissue were localized by immunohistochemistry. Tie-2 expression was measured by flow cytometry in circulating monocytes and infiltrated macrophages. Comparisons before and after sleeve gastrectomy were carried out using ANOVA for repeated measures. rs9930506FTO genotyping was performed by PCR-RFLP. Circulating sTie-2 and angiopoietin-2 were higher before sleeve gastrectomy. Tie-2 and angiopoietin-2 mRNA levels were higher in subcutaneous adipose tissue than visceral and both decreased after surgery. Monocytes and infiltrated macrophages showed a pro-inflammatory phenotype, with increased Tie-2 expression that decreased 3 and 6 months after sleeve gastrectomy. Baseline sTie-2 correlated inversely with adiponectin levels. At baseline the rs9930506 FTO AG ó GG genotypes carriers had more 34 kg than genotype carriers of rs9930506 AA. Weight and body mass index decreased at 6 months. We found that angiopoietin/Tie-2 system is mainly expressed in subcutaneous adipose tissue, contributing to expandability, fat accumulation, and monocytes attachment in obesity. Bariatric surgery favorably modifies the pro-angiogenic profile, allowed a reduced angiogenic expression in the circulation and adipose tissue.
ISSN:1355-008X
1559-0100
DOI:10.1007/s12020-016-1070-y