Occurrence and colocalization of surfactant proteins A, B, C and D in the developing and adult rat brain

Surfactant proteins (SP’s) have been described as inherent proteins of the human central nervous system (CNS). Their distribution pattern in brain tissue and altered cerebrospinal fluid (CSF) – concentrations in different CNS pathologies are indicative of their immunological and rheological importan...

Full description

Saved in:
Bibliographic Details
Published in:Annals of anatomy 2017-03, Vol.210, p.121-127
Main Authors: Schob, Stefan, Dieckow, Julia, Fehrenbach, Michael, Peukert, Nicole, Weiss, Alexander, Kluth, Dietrich, Thome, Ulrich, Quäschling, Ulf, Lacher, Martin, Preuß, Matthias
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Biomarkers
Blood brain barrier
Brain - growth & development
Brain Chemistry - physiology
Central nervous system
Cerebrospinal fluid
Development
Embryonic Development
Female
Glial Fibrillary Acidic Protein - metabolism
Immunohistochemistry
Lung
Occurence
Pregnancy
Pulmonary Surfactant-Associated Proteins - metabolism
Rats
Rats, Sprague-Dawley
Surfactant protein
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Surfactant proteins (SP’s) have been described as inherent proteins of the human central nervous system (CNS). Their distribution pattern in brain tissue and altered cerebrospinal fluid (CSF) – concentrations in different CNS pathologies are indicative of their immunological and rheological importance. The aim of this study has been to investigate when – compared to the lungs – SP’s are expressed in the developing rat brain and which functional components in the CNS participate in their production. Brain and lung tissue from embryonal (days 10, 12, 14, 16, 17 and 20), newborn, and adult rats were harvested and investigated for expression of SP-A, SP-B, SP-C and SP-D using immunofluorescence microscopy in order to identify and compare the time points of their occurence in the respective tissue. To better identify the location of SP expression in the rat brain, SP’s were colocalized with use of an astrocyte marker (GFAP), a neuronal marker (NeuN), an endothelial marker (CD31) and an axonal marker (NF). SP-A and SP-C are expressed in the CNS of rats during early embryonic age whereas SP-B and SP-D are first present in the adult rat brain. All SP’s are expressed in cells adjacent to CSF spaces, probably influencing and maintaining physiological CSF flow. SP’s A and C are abundant at the site of the blood brain barrier (BBB).
ISSN:0940-9602
1618-0402
DOI:10.1016/j.aanat.2016.10.006