Analysis and detection of the chemical constituents of Radix Polygalae and their metabolites in rats after oral administration by ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry

[Display omitted] •A UHPLC/ESI-Q-TOF-MS method was established for Radix Polygalae and its metabolism.•41 known and 9 new compounds were identified in Radix Polygalae within 19min.•34 components were detected in the drug-containing samples. Radix Polygalae (RP), the dried root of Polygala tenuifolia...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2013-11, Vol.85, p.1-13
Main Authors: Ling, Yun, Li, Zhixiong, Chen, Mingcang, Sun, Zhaolin, Fan, Mingsong, Huang, Chenggang
Format: Article
Language:English
Subjects:
acetonitrile
Administration, Oral
Animals
aqueous solutions
central nervous system
chemical composition
Chemical constituents
Chromatography, High Pressure Liquid - methods
cognition
Drugs, Chinese Herbal - analysis
Drugs, Chinese Herbal - metabolism
formic acid
ionization
Male
Metabolites
metabolomics
Oligosaccharides - analysis
oral administration
Oriental traditional medicine
Polygala
Polygala - chemistry
Polygala tenuifolia
Radix Polygalae
Rats
Rats, Sprague-Dawley
Saponins - analysis
sedatives
Spectrometry, Mass, Electrospray Ionization - methods
tandem mass spectrometry
Tandem Mass Spectrometry - methods
therapeutics
UHPLC/ESI-Q-TOF-MS
ultra-performance liquid chromatography
urine
Xanthones - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] •A UHPLC/ESI-Q-TOF-MS method was established for Radix Polygalae and its metabolism.•41 known and 9 new compounds were identified in Radix Polygalae within 19min.•34 components were detected in the drug-containing samples. Radix Polygalae (RP), the dried root of Polygala tenuifolia Willd., is a well-known traditional Chinese medicine to mediate sedative, antipsychotic, cognitive improving, neuroprotective, and anti-inflammatory therapeutic effects on the central nervous system. In this work, ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC/ESI-Q-TOF-MS/MS) was established for the separation and characterization of the chemical constituents in Radix Polygalae and their metabolites in rat plasma and urine after oral administration. Samples were separated on an Agilent Zorbax Eclipse Plus-C18 column (100mm×2.1mm, 1.8μm) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Overall, 50 compounds were characterized from the RP, 9 of which are to our knowledge reported for the first time. In vivo, 10 components and 2 metabolites were observed in rat plasma, and 27 components and 7 metabolites were detected in rat urine. The results from this work improve our understanding on the chemical constituents of RP and their metabolic profiling.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2013.06.011