Comparative proteomics reveals highly and differentially expressed proteins in field-collected and laboratory-cultured blooming cells of the diatom Skeletonema costatum

Summary Diatoms are a major phytoplankton group causing extensive blooms in the ocean. However, little is known about the intracellular biological processes occurring during the blooming period. This study compared the protein profiles of field‐collected and laboratory‐cultured blooming cells of Ske...

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Bibliographic Details
Published in:Environmental microbiology 2015-10, Vol.17 (10), p.3976-3991
Main Authors: Zhang, Hao, Wang, Da-Zhi, Xie, Zhang-Xian, Zhang, Shu-Fei, Wang, Ming-Hua, Lin, Lin
Format: Article
Language:English
Subjects:
Amino Acids - metabolism
Bacillariophyceae
Carbohydrate Metabolism - physiology
Carbon - metabolism
Cell division
Diatoms - genetics
Diatoms - metabolism
Energy Metabolism - physiology
Laboratories
Nitrogen - metabolism
Photosynthesis - genetics
Phytoplankton - metabolism
Proteins
Proteins - genetics
Proteomics
Proteomics - methods
Skeletonema costatum
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Summary:Summary Diatoms are a major phytoplankton group causing extensive blooms in the ocean. However, little is known about the intracellular biological processes occurring during the blooming period. This study compared the protein profiles of field‐collected and laboratory‐cultured blooming cells of Skeletonema costatum, and identified highly and differentially expressed proteins using the shotgun proteomic approach. A total of 1372 proteins were confidently identified with two or more peptides. Among them, 222 and 311 proteins were unique to the laboratory and field samples respectively. Proteins involved in photosynthesis, translation, nucleosome assembly, carbohydrate and energy metabolism dominated the protein profiles in both samples. However, different features of specific proteins were also found: proteins participated in light harvesting, photosynthetic pigment biosynthesis, photoprotection, cell division and redox homeostasis were highly detected in the field sample, whereas proteins involved in translation, amino acid and protein metabolic processes, and nitrogen and carbon assimilation presented high detection rates in the laboratory sample. ATP synthase cf1 subunit beta and light harvest complex protein were the most abundant protein in the laboratory and field samples respectively. These results indicated that S. costatum had evolved adaptive mechanisms to the changing environment, and integrating field and laboratory proteomic data should provide comprehensive understanding of bloom mechanisms.
ISSN:1462-2912
1462-2920
DOI:10.1111/1462-2920.12914