Arginase I and the very low-density lipoprotein receptor are associated with phenotypic biomarkers for obesity

Abstract Objective Obesity is a serious health problem implicated in many metabolic disorders (i.e., hypertension, dyslipidemia, and cardiovascular disease). We examined whether the mRNA of tested genes were linked to blood lipid concentrations and vascular endothelial function as features of obesit...

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Published in:Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2012-06, Vol.28 (6), p.635-639
Main Authors: Kim, Oh Yoen, Ph.D, Lee, Seung-Min, Ph.D, Chung, Ji Hyung, Ph.D, Do, Hyun Joo, M.S, Moon, Jiyoung, M.S, Shin, Min-Jeong, Ph.D
Format: Article
Language:English
Subjects:
Adiponectin - blood
Adult
Aged
Apolipoproteins B - blood
Arginase - genetics
Arginase - metabolism
Arginase I
Biological and medical sciences
Biomarkers - blood
Blood Glucose - metabolism
Body Mass Index
Cardiovascular disease
Cardiovascular risk
Cholesterol
Cholesterol - blood
Endothelium, Vascular
Feeding. Feeding behavior
Female
Fundamental and applied biological sciences. Psychology
Gastroenterology and Hepatology
Gene Expression
Humans
Insulin resistance
Intercellular Adhesion Molecule-1 - blood
Leukocytes, Mononuclear - metabolism
Male
Middle Aged
Obesity
Obesity - blood
Obesity - genetics
Obesity - metabolism
Phenotype
Proteins
Receptors, LDL - genetics
Receptors, LDL - metabolism
RNA, Messenger - metabolism
Soluble intercellular adhesion molecule-1
Studies
Triglycerides - blood
Up-Regulation
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Very low-density lipoprotein receptor
Weight control
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Summary:Abstract Objective Obesity is a serious health problem implicated in many metabolic disorders (i.e., hypertension, dyslipidemia, and cardiovascular disease). We examined whether the mRNA of tested genes were linked to blood lipid concentrations and vascular endothelial function as features of obesity. Methods In healthy subjects (30–69 y old; normal weight, n = 22, body mass index 18.5–23 kg/m2 ; overweight, n = 25, body mass index ≥23 kg/m2 ) the following parameters were measured in the blood circulation: total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, triacylglycerol, apolipoprotein AI, apolipoprotein B, adiponectin, high-sensitivity C-reactive protein, and soluble intercellular adhesion molecule-1. The mRNA levels of genes (arginase I [ ARG1 ], very low-density lipoprotein receptor [ VLDLR ], adiponectin receptor-1 [ ADIPOR1 ], ADIPOR2 , and nitric oxide synthase-3 [ NOS3 ]) were tested in the subjects’ peripheral blood mononuclear cells. Results The expression levels of all tested genes were investigated for their associations with the blood concentrations of each parameter. In the expression study, only ARG1 (4.5-fold) and VLDLR (4-fold) expressions were significantly upregulated in the overweight group compared with the normal-weight group. The ARG1 mRNA levels were positively associated with blood concentrations of total cholesterol, low-density lipoprotein cholesterol, apolipoprotein B, and soluble intercellular adhesion molecule-1. The VLDLR mRNA levels showed a positive relation with triacylglycerol and glucose concentrations and a negative relation with adiponectin levels. Conclusion Significant upregulations of ARG1 and VLDLR were observed in the overweight condition and their expression levels are likely to be closely linked to the phenotypic biomarkers for obesity (disturbed lipid profiles and endothelial dysfunction).
ISSN:0899-9007
1873-1244
DOI:10.1016/j.nut.2011.09.012