Lactate Formation in Primary and Metastatic Colon Cancer Cells at Hypoxia and Normoxia

High glucose consumption and lactate synthesis in aerobic glycolysis are a hallmark of cancer cells. They can form lactate also in glutaminolysis, but it is not clear how oxygen availability affects this process. We studied lactate synthesis at various oxygen levels in human primary (SW480) and meta...

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Bibliographic Details
Published in:Cell biochemistry and function 2016-10, Vol.34 (7), p.483-490
Main Authors: Graboń, Wojciech, Otto-Ślusarczyk, Dagmara, Chrzanowska, Alicja, Mielczarek-Puta, Magdalena, Joniec-Maciejak, Ilona, Słabik, Krzysztof, Barańczyk-Kuźma, Anna
Format: Article
Language:English
Subjects:
Amino acids
aspartate aminotransferases
Aspartate Aminotransferases - genetics
Aspartate Aminotransferases - metabolism
Aspartic Acid - pharmacology
Cancer
Cell Count
Cell Hypoxia - drug effects
Cell Line, Tumor
Colon
Colon cancer
colon cancer cell lines
Colonic Neoplasms - enzymology
Colonic Neoplasms - genetics
Colonic Neoplasms - metabolism
Colonic Neoplasms - pathology
Colorectal cancer
Colorimetry
Culture Media - chemistry
expression
Gene expression
Gene Expression Regulation, Neoplastic - drug effects
Glucose
Glucose - metabolism
Glycolysis
High-performance liquid chromatography
Humans
Hypoxia
L-aspartate
L-serine
lactate synthesis
Lactic acid
Lactic Acid - metabolism
Liquid chromatography
Metastases
Metastasis
Models, Biological
Neoplasm Metastasis
Oxygen
Oxygen - pharmacology
Polymerase chain reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Serine - pharmacology
Synthesis
tissue normoxia
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Summary:High glucose consumption and lactate synthesis in aerobic glycolysis are a hallmark of cancer cells. They can form lactate also in glutaminolysis, but it is not clear how oxygen availability affects this process. We studied lactate synthesis at various oxygen levels in human primary (SW480) and metastatic (SW620) colon cancer cells cultured with L‐Ser and/or L‐Asp. Glucose and lactate levels were determined colorimetrically, amino acids by HPLC, expression of AST1‐mRNA and AST2‐mRNA by RT‐PCR. In both lines glucose consumption and lactate synthesis were higher at 10% than at 1% oxygen, and lactate/glucose ratio was increased above 2.0 by L‐Asp. AST1‐mRNA expression was independent on oxygen and cell line, but AST2‐mRNA was lower at hypoxia in SW480. We conclude that, in both cell lines at 1% hypoxia, lactate is formed mainly from glucose but at 10% normoxia also from L‐Asp. At 10% normoxia, lactate synthesis is more pronounced in primary than metastatic colon cancer cells.
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.3211