Type of chromosome abnormality affects embryo morphology dynamics

Objective To study the differences in the cleavage time between types of embryo chromosomal abnormalities and elaborate algorithm to exclude aneuploid embryos according to the likelihood to be euploid. Design Retrospective cohort study. Setting University affiliated private center. Patient(s) Preimp...

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Bibliographic Details
Published in:Fertility and sterility 2017-01, Vol.107 (1), p.229-235.e2
Main Authors: Del Carmen Nogales, Maria, Ph.D, Bronet, Fernando, Ph.D, Basile, Natalia, Ph.D, Martínez, Eva María, Ph.D, Liñán, Alberto, Ph.D, Rodrigo, Lorena, Ph.D, Meseguer, Marcos, Ph.D
Format: Article
Language:English
Subjects:
Adult
Aneuploidy
array CGH
Biopsy
Blastocyst - pathology
Chromosome Aberrations
Chromosome Disorders - diagnosis
Chromosome Disorders - genetics
Chromosome Disorders - pathology
Chromosomes, Human
Comparative Genomic Hybridization
complex abnormalities
Embryo kinetics
Embryonic Development
Female
Fertilization in Vitro - adverse effects
Genetic Testing
Humans
Internal Medicine
Kinetics
Logistic Models
Microscopy, Video
Obstetrics and Gynecology
Odds Ratio
Predictive Value of Tests
Pregnancy
Preimplantation Diagnosis - methods
Retrospective Studies
Risk Factors
time lapse
Time-Lapse Imaging - methods
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Summary:Objective To study the differences in the cleavage time between types of embryo chromosomal abnormalities and elaborate algorithm to exclude aneuploid embryos according to the likelihood to be euploid. Design Retrospective cohort study. Setting University affiliated private center. Patient(s) Preimplantational genetic screening patients (n = 112) including cases of advanced maternal age, repeated implantation failure, and recurrent miscarriage. A total of 485 embryos were analyzed. Intervention(s) None. Main Outcome Measure(s) All biopsied embryos were cultured in an incubator with time-lapse technology, cleavage timing from insemination to day 3 and all kinetic parameters that have been described in previous studies by our group. Result(s) Logistic regression analysis were used to identify morphokinetic parameters and some were strongly associated with complex aneuploid embryos; t3 (odds ratio = 0.590, 95% confidence interval 0.359–0.971) and t5–t2 (odds ratio = 0.151, 95% confidence interval 0.082–0.278). Conclusion(s) Embryo morphokinetics are affected by chromosome aneuploidy and further analysis of the chromosome content reveals higher differences when the complexity in the chromosome disorders is increased. The use of time-lapse monitoring, although not able to detect an abnormal embryo, may be potentially useful to discard those embryos with high risk of complex chromosomal abnormalities.
ISSN:0015-0282
1556-5653
DOI:10.1016/j.fertnstert.2016.09.019