Viability and growth of feline preantral follicles in vitro cultured with insulin growth factor and epidermal growth factor supplemented medium

Contents In vitro culture of ovarian preantral follicles has emerged as a reproductive technology aimed at obtaining large amount of oocytes for in vitro embryo production. The addition of growth factors (GF) in the in vitro culture of preantral follicles of different species has provided superior r...

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Bibliographic Details
Published in:Reproduction in domestic animals 2017-04, Vol.52 (S2), p.93-97
Main Authors: Alves, AE, Padilha‐Nakaghi, LC, Pires‐Butler, EA, Apparicio, M, Silva, NAM, Motheo, TF, Vicente, WRR, Luvoni, GC
Format: Article
Language:English
Subjects:
Animals
cat
Cats - physiology
Cells, Cultured
Culture Media - pharmacology
Epidermal Growth Factor - pharmacology
Female
Follicle Stimulating Hormone - pharmacology
follicles
Granulosa Cells - cytology
Granulosa Cells - drug effects
in vitro development
Insulin-Like Growth Factor I - pharmacology
Oocytes - cytology
Oocytes - drug effects
Ovarian Follicle - drug effects
Ovarian Follicle - growth & development
Tissue Culture Techniques - veterinary
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Summary:Contents In vitro culture of ovarian preantral follicles has emerged as a reproductive technology aimed at obtaining large amount of oocytes for in vitro embryo production. The addition of growth factors (GF) in the in vitro culture of preantral follicles of different species has provided superior results of follicular development, antrum formation and proliferation of granulosa cells. However, there are only few reports regarding the use of these factors on feline preantral follicle in vitro culture. Thus, the aim of this study was to investigate the effect of a combination of IGF‐1 and EGF on in vitro viability and growth of preantral follicles and enclosed oocytes collected from domestic cats. A total of 64 follicles characterized by multilayer granulosa cells were isolated and individually cultured for 6 days (T6) in minimum essential medium supplemented with IGF‐1+ EGF (100 ng/ml each) or without (control). A higher percentage of follicles were viable after culture with GF than without, and an increase in size when IGF‐1+ EGF were added to the medium (170 ± 32.4 μm (T0) vs. 201 ± 22.3 μm (T6); p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12839