Cotton Stalk Pretreatment Using Daedalea flavida, Phlebia radiata, and Flavodon flavus: Lignin Degradation, Cellulose Recovery, and Enzymatic Saccharification

Lignocellulolytic enzyme activities of selective fungi Daedalea flavida MTCC 145 (DF-2), Phlebia radiata MTCC 2791 (PR), and non-selective fungus Flavodon flavus MTCC 168 (FF) were studied for pretreatment of cotton stalks. Simultaneous productions of high LiP and laccase activities by DF-2 during e...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2017-04, Vol.181 (4), p.1465-1484
Main Authors: Meehnian, Harmanpreet, Jana, Asim K
Format: Article
Language:English
Subjects:
Biochemistry
Biomass
Biotechnology
Biotechnology - methods
Cellulose
Cellulose - metabolism
Chemistry
Chemistry and Materials Science
Cotton
Enzymes
Enzymes - metabolism
Gossypium - chemistry
Lignin
Lignin - metabolism
Lignocellulose
Polyporales - enzymology
Polyporales - growth & development
Polyporales - metabolism
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Summary:Lignocellulolytic enzyme activities of selective fungi Daedalea flavida MTCC 145 (DF-2), Phlebia radiata MTCC 2791 (PR), and non-selective fungus Flavodon flavus MTCC 168 (FF) were studied for pretreatment of cotton stalks. Simultaneous productions of high LiP and laccase activities by DF-2 during early phase of growth were effective for lignin degradation 27.83 ± 1.25 % ( w / w of lignin) in 20-day pretreatment. Production of high MnP activity without laccase in the early growth phase of PR was ineffective and delayed lignin degradation 24.93 ± 1.53 % in 25 days due to laccase production at later phase. With no LiP activity, low activities of MnP and laccase by FF yielded poor lignin degradation 15.09 ± 0.6 % in 20 days. Xylanase was predominant cellulolytic enzyme produced by DF-2, resulting hemicellulose as main carbon and energy source with 83 % of cellulose recovery after 40 days of pretreatment. The glucose yield improved more than two fold from 20-day DF-2 pretreated cotton stalks after enzymatic saccharification.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-016-2296-0