Shotgun Lipidomic Profiling of the NCI60 Cell Line Panel Using Rapid Evaporative Ionization Mass Spectrometry

Rapid evaporative ionization mass spectrometry (REIMS) was used for the rapid mass spectrometric profiling of cancer cell lines. Spectral reproducibility was assessed for three different cell lines, and the extent of interclass differences and intraclass variance was found to allow the identificatio...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2016-08, Vol.88 (15), p.7507-7514
Main Authors: Strittmatter, Nicole, Lovrics, Anna, Sessler, Judit, McKenzie, James S., Bodai, Zsolt, Doria, M. Luisa, Kucsma, Nora, Szakacs, Gergely, Takats, Zoltan
Format: Article
Language:English
Subjects:
Bacterial infections
Biotechnology
Cancer
Cell Line, Tumor - microbiology
Cells
Evaporative
Humans
Ionization
Lipids
Lipids - analysis
Mass spectrometry
Mass Spectrometry - methods
Mycoplasma
Neoplasms - metabolism
Panels
Principal Component Analysis
Profiling
Reproducibility of Results
Spectral lines
Tissues
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Summary:Rapid evaporative ionization mass spectrometry (REIMS) was used for the rapid mass spectrometric profiling of cancer cell lines. Spectral reproducibility was assessed for three different cell lines, and the extent of interclass differences and intraclass variance was found to allow the identification of these cell lines based on the REIMS data. Subsequently, the NCI60 cell line panel was subjected to REIMS analysis, and the resulting data set was investigated for its distinction of individual cell lines and different tissue types of origin. Information content of REIMS spectral profiles of cell lines were found to be similar to those obtained from mammalian tissues although pronounced differences in relative lipid intensity were observed. Ultimately, REIMS was shown to detect changes in lipid content of cell lines due to mycoplasma infection. The data show that REIMS is an attractive means to study cell lines involving minimal sample preparation and analysis times in the range of seconds.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.6b00187