Effect of Concentrated Growth Factor on Survival of Diced Cartilage Graft

Background Diced cartilage grafts are important in rhinoplasty for raising the dorsum and eliminating dorsal irregularities. The most common problems with the use of diced cartilage are wrapping and cartilage resorption. Objectives To histopathologically investigate and compare the viability of dice...

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Bibliographic Details
Published in:Aesthetic surgery journal 2016-11, Vol.36 (10), p.1176-1187
Main Authors: Topkara, Adem, Özkan, Adem, Özcan, Ramazan Hakan, Öksüz, Mustafa, Akbulut, Metin
Format: Article
Language:English
Subjects:
Animals
Back Muscles - transplantation
Cartilage - drug effects
Cartilage - pathology
Cartilage - transplantation
Fibrin Tissue Adhesive
Graft Survival - drug effects
Intercellular Signaling Peptides and Proteins - administration & dosage
Intercellular Signaling Peptides and Proteins - blood
Male
Rabbits
Time Factors
Tissue and Organ Harvesting - methods
Tissue Survival - drug effects
Transplantation, Autologous
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Summary:Background Diced cartilage grafts are important in rhinoplasty for raising the dorsum and eliminating dorsal irregularities. The most common problems with the use of diced cartilage are wrapping and cartilage resorption. Objectives To histopathologically investigate and compare the viability of diced cartilage grafts wrapped with concentrated growth factor, fascia and fenestrated fascia, or blood glue. Methods Cartilage grafts were harvested from the ears of 10 New Zealand White rabbits and diced into 0.5 to 1 mm3 pieces. The grafts were divided into five groups for comparison: (1) bare diced cartilage; (2) diced cartilage wrapped with fascia; (3) diced cartilage wrapped with fenestrated fascia; (4) diced cartilage wrapped with concentrated growth factor (CGF); and (5) diced cartilage wrapped with blood glue. Each of the five grafts was autologously implanted into a subcutaneous pocket in the back of each rabbit. Three months later, the rabbits were sacrificed and the implants were harvested and examined histopathologically. Results Nucleus loss, calcification, inflammation, and giant cell formation differed significantly between the CGF group and both fascia groups. Chondrocyte proliferation was the highest in the CGF group. Nucleus loss rates were similar between the fascia and fenestrated fascia groups. Conclusions Our findings suggest that CGF improves the viability of diced cartilage grafts, while fascia hampers it. Punching holes in the fascia does not improve diced cartilage graft viability and neither does blood glue wrapping.
ISSN:1090-820X
1527-330X
DOI:10.1093/asj/sjw137