The mixture of liquid foam soap, ethanol and citric acid as a new fixative–preservative solution in veterinary anatomy

The present study investigates the efficiency of liquid foam soap, ethanol, citric acid and benzalkonium chloride as a fixative–preservative solution (a soap-and ethanol-based fixing solution, or SEFS). In this study, ethanol serves as the fixative and preservative, liquid foam soap as the modifying...

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Bibliographic Details
Published in:Annals of anatomy 2017-01, Vol.209, p.11-17
Main Authors: Turan, Erkut, Gules, Ozay, Kilimci, Figen Sevil, Kara, Mehmet Erkut, Dilek, Omer Gurkan, Sabanci, Seyyid Said, Tatar, Musa
Format: Article
Language:English
Subjects:
Anatomy, Veterinary - methods
Animals
Cadaver
Citric acid
Citric Acid - chemistry
Complex Mixtures - chemical synthesis
Embalming
Embalming - methods
Ethanol
Ethanol - chemistry
Fixative
Fixatives - chemical synthesis
Gases - chemistry
Goats - anatomy & histology
Liquid foam soap
Materials Testing
Organ Preservation Solutions - chemical synthesis
Preservative
Soaps - chemistry
Solutions
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Summary:The present study investigates the efficiency of liquid foam soap, ethanol, citric acid and benzalkonium chloride as a fixative–preservative solution (a soap-and ethanol-based fixing solution, or SEFS). In this study, ethanol serves as the fixative and preservative, liquid foam soap as the modifying agent, citric acid as the antioxidant and benzalkonium chloride as the disinfectant. The goat cadavers perfused with SEFS (n=8) were evaluated over a period of one year with respect to hardness, colour and odour using objective methods. Colour and hardness were compared between one fresh cadaver and the SEFS-embalmed cadavers. Histological and microbiological examinations were also performed in tissue samples. Additionally, the cadavers were subjectively evaluated after dissection and palpation. The SEFS provided the effectiveness expected over a 1-year embalming period for the animal cadavers. No bacteria or fungi were isolated except for some non-pathogenic Bacillus species. Visible mould was not present on either cadavers or in the surrounding environment. The cadavers maintained an appearance close to their original anatomical appearance, with muscles having good hardness and elasticity for dissection.
ISSN:0940-9602
1618-0402
DOI:10.1016/j.aanat.2016.09.002