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Communities of endophytic microorganisms in different developmental stages from a local variety as well as transgenic and conventional isogenic hybrids of maize
The diversity of endophytic microorganisms may change due to the genotype of the host plant and its phenological stage. In this study we evaluated the effect of phenological stage, transgenes and genetic composition of maize on endophytic bacterial and fungal communities. The maize populations were...
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Published in: | World journal of microbiology & biotechnology 2016-11, Vol.32 (11), p.189-189, Article 189 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The diversity of endophytic microorganisms may change due to the genotype of the host plant and its phenological stage. In this study we evaluated the effect of phenological stage, transgenes and genetic composition of maize on endophytic bacterial and fungal communities. The maize populations were composed of a local variety named Rosado (
RS
) and three isogenic hybrids. One isogenic hybrid was not genetically modified (
NGM
). Another hybrid (
Hx
) contained the transgenes
cry1F
and
pat
(T1507 event), which provide resistance to insects of the order
Lepidoptera
and tolerance to the glufosinate-ammonium herbicide, respectively. The third hybrid (
Hxrr
) contained the transgene
cp4 epsps
(NK603 event) combined with the transgenes
cry1F
and
pat
(T1507 event), which allow tolerance to the Roundup Ready herbicide, besides the characteristics of
Hx
. Evaluation of the foliar tissue was done through PCR-DGGE analysis, with specific primers for bacteria and fungi within four phenological stages of maize. The endophytic bacteria were only clustered by phenological stages; the structure of the fungal community was clustered by maize genotypes in each phenological stage. The fungal community from the local variety
RS
was different from the three hybrids (
NGM
,
Hx
and
Hxrr
) within the four evaluated stages. In the reproductive stage, the fungal community from the two transgenic hybrids (
Hx
and
Hxrr
) were separated, and the
Hxrr
was different from
NGM
, in the two field experiments. This research study showed that the genetic composition of the maize populations, especially the presence of transgenes, is the determining factor for the changes detected in the endophytic fungal community of maize leaves. |
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ISSN: | 0959-3993 1573-0972 |
DOI: | 10.1007/s11274-016-2149-6 |