Influence of cyanoacrylate on the efficiency of forensic PCRs

Cyanoacrylate ester (CA) is commonly used by criminalists to detect latent fingerprints on smooth surfaces. We investigated whether this treatment has an influence on a subsequent DNA typing of biological stains, and on the efficiency of three different forensic PCRs (mtDNA, Y-STR determination and...

Full description

Saved in:
Bibliographic Details
Published in:Forensic science international 2001-12, Vol.124 (1), p.11-16
Main Authors: von Wurmb, Nicole, Meissner, Diane, Wegener, Rudolf
Format: Article
Language:English
Subjects:
AbiPrism310
Amplification
Automation
Biological and medical sciences
Cyanoacrylate ester
Cyanoacrylates - adverse effects
Deoxyribonucleic acid
Diverse techniques
DNA
DNA Fingerprinting - methods
DNA fingerprints
Efficiency
Experiments
Fingerprinting
Fluorescence
Forensic
Forensic Medicine
Forensic PCR
Forensic science
Forensic sciences
Fundamental and applied biological sciences. Psychology
Humans
Methods
Mitochondrial DNA
Molecular and cellular biology
mtDNA
Nucleic Acid Amplification Techniques
PCR efficiency
Polymerase Chain Reaction - methods
Primers
Saliva
Stains & staining
STR typing
Typing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cyanoacrylate ester (CA) is commonly used by criminalists to detect latent fingerprints on smooth surfaces. We investigated whether this treatment has an influence on a subsequent DNA typing of biological stains, and on the efficiency of three different forensic PCRs (mtDNA, Y-STR determination and the Profiler Plus kit). Using fluorescence labeled primers and an automated detection system, we could show that the presence of CA led to weaker PCR products. Depending on the DNA extraction method the amplification results were significantly weaker compared to untreated controls. To simulate forensic cases we prepared blood and saliva stains on glass slides, extracted the DNA using two different methods and compared the signal intensities of the amplified DNA fragments. Depending on the extraction methods, the presence of CA significantly hampered the amplification of DNA from small stains whereas there was virtually no difference comparing the amplification results of DNA extracted from bigger stains.
ISSN:0379-0738
1872-6283
DOI:10.1016/S0379-0738(01)00515-1