Identification and characterization of toxigenic Bacillus cereus isolates responsible for two food-poisoning outbreaks

The epidemiology of Bacillus cereus strains responsible for food poisoning is scantly known, mostly because the genotypic and toxigenic properties of the B. cereus strains isolated during food-poisoning outbreaks have been never catalogued. The occurrence of two simultaneous food-poisoning outbreaks...

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Bibliographic Details
Published in:FEMS microbiology letters 2002-02, Vol.208 (1), p.129-134
Main Authors: Ghelardi, E, Celandroni, F, Salvetti, S, Barsotti, C, Baggiani, A, Senesi, S
Format: Article
Language:English
Subjects:
Bacillus cereus
Bacillus cereus - classification
Bacillus cereus - genetics
Bacillus cereus - isolation & purification
Bacillus cereus - pathogenicity
Bacterial Proteins - genetics
Bacterial Proteins - toxicity
Bacterial Toxins - genetics
Bacterial Toxins - metabolism
Bacterial Toxins - toxicity
Bacteriology
Biological and medical sciences
Cereulide
cytotoxins
Deoxyribonucleic acid
Depsipeptides
Disease Outbreaks
DNA
Emetic toxin
Enterotoxin
Enterotoxins
Enterotoxins - genetics
Enterotoxins - metabolism
Enterotoxins - toxicity
epidemiological studies
Epidemiology
Food
Food contamination
Food poisoning
Foodborne Diseases - epidemiology
Foodborne Diseases - microbiology
foodborne illness
Fundamental and applied biological sciences. Psychology
genes
Genetics
Hemolysin
Hemolysin Proteins
hemolysins
Humans
Lecithin
Microbiology
Multiplex random amplified polymorphic DNA‐PCR
Outbreaks
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Peptides, Cyclic - genetics
Peptides, Cyclic - metabolism
Peptides, Cyclic - toxicity
Phosphatidylcholine
Phosphatidylinositol
Phospholipase
Phospholipase C
Poisoning
Polymerase chain reaction
Random amplified polymorphic DNA
Random Amplified Polymorphic DNA Technique
Random amplified polymorphic DNA‐PCR
Sphingomyelin phosphodiesterase
sphingomyelinase
Strains (organisms)
Type C Phospholipases - genetics
Type C Phospholipases - metabolism
Type C Phospholipases - toxicity
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Summary:The epidemiology of Bacillus cereus strains responsible for food poisoning is scantly known, mostly because the genotypic and toxigenic properties of the B. cereus strains isolated during food-poisoning outbreaks have been never catalogued. The occurrence of two simultaneous food-poisoning outbreaks gave us the opportunity to wonder whether (i) the identity of individual strains isolated from clinical, environmental, and food samples could be established by random amplified polymorphic DNA (RAPD)-PCR and multiplex RAPD-PCR, and (ii) the toxigenic potential of the isolates could be determined by testing their ability to secrete hemolysin BL, phosphatidylcholine-specific phospholipase C, and cereulide, as well as by determining the presence of the genes encoding enterotoxins NHE, T, and FM/S, cytotoxin K, sphingomyelinase, and phosphatidylinositol-specific phospholipase C. This is the first report demonstrating that the combination of several phenotypic and genotypic traits provides a powerful tool for tracing the source of infection of toxigenic B. cereus strains relevant for epidemiological survey.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2002.tb11072.x