EVALUATION OF A PCR-BASED METHOD TO DETECT VERTICILLIUM DAHLIAE IN CHICORY TAPROOTS

The identification of red chicory plants infected by Verticillium dahliae at the moment of transplant for seed production is needed since at this time some infected plants could be symptomless. Since the usual plating method is time-consuming and not always reliable, a PCR-based method was evaluated...

Full description

Saved in:
Bibliographic Details
Published in:Journal of plant pathology 2001-11, Vol.83 (3), p.221-224
Main Authors: Mascarello, N., Favaron, F., Di Lenna, P.
Format: Article
Language:English
Subjects:
Annealing
Cichorium intybus
Gels
Pathogens
Phytopathology
Plant diseases
Plants
Polymerase chain reaction
Product category rules
Seed production
Short Communication
Verticillium
Verticillium dahliae
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The identification of red chicory plants infected by Verticillium dahliae at the moment of transplant for seed production is needed since at this time some infected plants could be symptomless. Since the usual plating method is time-consuming and not always reliable, a PCR-based method was evaluated, using oligonucleotide primers described as specific for this pathogen. The experiments allowed to obtain the optimal amplification profile and to verify the good specificity of the primers VMSP1/VMSP2 that, with DNA extracted from Verticillium-infected taproot tissues, amplify a specific fragment of 140 bp.
ISSN:1125-4653
2239-7264