Mapping and validation of Xanthomonas citri subsp citri genes regulated by putative plant-inducible promoter box (PIP-box)

Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp citri (Xac), is a major disease affecting citriculture worldwide, because of the susceptibility of the host and the lack of efficient control methods. Previous studies have reported that some genes of phytopathogenic bacter...

Full description

Saved in:
Bibliographic Details
Published in:Genetics and molecular research 2016-05, Vol.15 (2)
Main Authors: Carvalho, F M S, Oliveira, J C F, Laia, M L, Jacob, T R, Ferreira, R M, Ferro, M I T, Tezza, R I D, Zingaretti, S M, Silva, C F, Ferro, J A
Format: Article
Language:English
Subjects:
Citrus
Gene Expression Regulation, Bacterial
Genes, Bacterial
Physical Chromosome Mapping
Promoter Regions, Genetic
Virulence - genetics
Virulence Factors - genetics
Virulence Factors - metabolism
Xanthomonas
Xanthomonas - genetics
Xanthomonas - pathogenicity
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp citri (Xac), is a major disease affecting citriculture worldwide, because of the susceptibility of the host and the lack of efficient control methods. Previous studies have reported that some genes of phytopathogenic bacteria possess a consensus nucleotide sequence (TTCGC...N15...TTCGC) designated the "plant-inducible-promoter box" (PIP box) located in the promoter region, which is responsible for activating the expression of pathogenicity and virulence factors when the pathogen is in contact with the host plant. In this study, we mapped and investigated the expression of 104 Xac genes associated with the PIP box sequences using a macroarray analysis. Xac gene expression was observed during in vitro (Xac grown for 12 or 20 h in XAM1 induction medium) or in vivo (bacteria grown in orange leaves for 3 to 5 days) infection conditions. Xac grown in non-induction NB liquid medium was used as the control. cDNA was isolated from bacteria grown under the different conditions and hybridized to the macroarray, and 32 genes differentially expressed during the infection period (in vitro or in vivo induction) were identified. The macroarray results were validated for some of the genes through semi-quantitative RT-PCR, and the functionality of the PIP box-containing promoter was demonstrated by activating b-glucuronidase reporter gene activity by the PIP box-containing promoter region during Xac-citrus host interaction.
ISSN:1676-5680
1676-5680
DOI:10.4238/gmr.15028428