Air pollution-related metals induce differential cytokine responses in bronchial epithelial cells

Different transition metals have been shown to induce inflammatory responses in lung. We have compared eight different metal ions with regard to cytokine responses, cytotoxicity and signalling mechanisms in a human lung epithelial cell model (BEAS-2B). Among the metal ions tested, there were large d...

Full description

Saved in:
Bibliographic Details
Published in:Toxicology in vitro 2016-10, Vol.36, p.53-65
Main Authors: Låg, M, Øvrevik, J, Totlandsdal, A I, Lilleaas, E M, Thormodsæter, A, Holme, J A, Schwarze, P E, Refsnes, M
Format: Article
Language:English
Subjects:
Air Pollutants - toxicity
Air Pollution - adverse effects
Arsenic - toxicity
Bronchi - cytology
Cell Line
Cell Survival - drug effects
Cytokines - genetics
Cytokines - metabolism
Dual Oxidases
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Gene Expression Regulation - drug effects
Humans
Metals - toxicity
NADPH Oxidases - genetics
NF-kappa B - metabolism
p38 Mitogen-Activated Protein Kinases - metabolism
Reactive Oxygen Species - metabolism
RNA, Messenger - metabolism
RNA, Small Interfering - genetics
Transcription Factor RelA - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Different transition metals have been shown to induce inflammatory responses in lung. We have compared eight different metal ions with regard to cytokine responses, cytotoxicity and signalling mechanisms in a human lung epithelial cell model (BEAS-2B). Among the metal ions tested, there were large differences with respect to pro-inflammatory potential. Exposure to Cd(2+), Zn(2+) and As(3+) induced CXCL8 and IL-6 release at concentrations below 100μM, and Mn(2+) and Ni(2+) at concentrations above 200μM. In contrast, VO4(3-), Cu(2+) and Fe(2+) did not induce any significant increase of these cytokines. An expression array of 20 inflammatory relevant genes also showed a marked up-regulation of CXCL10, IL-10, IL-13 and CSF2 by one or more of the metal ions. The most potent metals, Cd(2+), Zn(2+) and As(3+) induced highest levels of oxidative activity, and ROS appeared to be central in their CXCL8 and IL-6 responses. Activation of the MAPK p38 seemed to be a critical mediator. However, the NF-κB pathway appeared predominately to be involved only in Zn(2+)- and As(3+)-induced CXCL8 and IL-6 responses. Thus, the most potent metals Cd(2+), Zn(2+) and As(3+) seemed to induce a similar pattern for the cytokine responses, and with some exceptions, via similar signalling mechanisms.
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2016.07.004