In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines

The aim of this study was to compare the effects of cationic micelle and liposome drug delivery systems on liver and lung cells in a toxicological in vitro screening model, with observations on cytotoxicity and genotoxicity. A screening battery was established for assessment of a broad range of para...

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Bibliographic Details
Published in:Toxicology in vitro 2016-10, Vol.36, p.164-171
Main Authors: Roursgaard, Martin, Knudsen, Kristina Bram, Northeved, Helle, Persson, Mikael, Christensen, Tina, Kumar, Pramod E K, Permin, Anders, Andresen, Thomas L, Gjetting, Torben, Lykkesfeldt, Jens, Vesterdal, Lise K, Loft, Steffen, Møller, Peter
Format: Article
Language:English
Subjects:
A549 Cells
Cell Survival - drug effects
Cholesterol - toxicity
Drug Delivery Systems
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Fatty Acids, Monounsaturated - toxicity
Hep G2 Cells
Hepatocytes - drug effects
Humans
Interleukin-6 - metabolism
Liposomes - toxicity
Lung - cytology
Micelles
Polyethylene Glycols - toxicity
Polymethacrylic Acids - toxicity
Quaternary Ammonium Compounds - toxicity
Tumor Necrosis Factor-alpha - metabolism
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Summary:The aim of this study was to compare the effects of cationic micelle and liposome drug delivery systems on liver and lung cells in a toxicological in vitro screening model, with observations on cytotoxicity and genotoxicity. A screening battery was established for assessment of a broad range of parameters related to adverse effects. Clear concentration response effects were observed related to impairment of mitochondrial function, membrane integrity and oxidative stress markers, but no effect was observed on genotoxicity. The adverse effects were highest for the liposomes. The High Content Screening seems optimal for initial screening of adverse effects, and combined with standard cytotoxicity measurements initial screening can be performed for predictive toxicological screening.
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2016.08.002