High FABP5 versus CRABPII expression ratio in recurrent craniopharyngiomas: Implications for future treatment

Abstract Background and objective Recurrence is a major problem in craniopharyngioma (CP) management. Recent study shows that high FABP5/CRABPII may be related to tumor growth and Al-trans retinoic acid (ATRA) may suppress primary CP growth. We intended to study the expression profile of FABP5 and C...

Full description

Saved in:
Bibliographic Details
Published in:World neurosurgery 2016-10, Vol.94, p.197-205
Main Authors: Li, Qiang, M.D, You, Chao, M.D, Zhou, Liangxue, M.D, Sima, Xiutian, M.D, Liu, Zhiyong, M.D, Liu, Hao, M.D, Xu, Jianguo, M.D
Format: Article
Language:English
Subjects:
Adolescent
Adult
Apoptosis
Biomarkers, Tumor - metabolism
Child
Child, Preschool
China - epidemiology
CRABPII
Craniopharyngioma
Craniopharyngioma - diagnosis
Craniopharyngioma - epidemiology
Craniopharyngioma - metabolism
FABP5
Fatty Acid-Binding Proteins - metabolism
Female
Gene Expression Regulation, Neoplastic
Humans
Male
Middle Aged
Neoplasm Recurrence, Local - diagnosis
Neoplasm Recurrence, Local - epidemiology
Neoplasm Recurrence, Local - metabolism
Neurosurgery
Pituitary Neoplasms - diagnosis
Pituitary Neoplasms - epidemiology
Pituitary Neoplasms - metabolism
Prognosis
Receptors, Retinoic Acid - metabolism
Reproducibility of Results
Retinoic acid
Sensitivity and Specificity
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Background and objective Recurrence is a major problem in craniopharyngioma (CP) management. Recent study shows that high FABP5/CRABPII may be related to tumor growth and Al-trans retinoic acid (ATRA) may suppress primary CP growth. We intended to study the expression profile of FABP5 and CRABPII in recurrent CP tissue and the effect of ATRA on recurrent CP cells. Methods Fifty cases of CP patients were enrolled in the retrospective study. Among them, 15 were recurrent. Fresh specimens were collected for immunohistochemistry (IHC), RT-PCR and Western-blotting analysis of FABP5 and CRABPII. Fresh specimens from 6 primary and recurrent CPs were collected and subjected to cell culture using explants method. ATRA at various concentrations was applied to recurrent CP cell culture, and cell growth were recorded and analyzed. Results IHC, RT-PCR and Western-blot study showed that FABP5 was expressed significantly higher in recurrent tumors , while CRABPII in primary ones. FABP5/CRABPII ratio was significantly higher in recurrent rather than primary tumors. Recurrent CP cell grew faster than primary cells, and ATRA induced cellular apoptosis and inhibited CP cell growth in dose dependent manner. Conclusions High expression ratio between FABP5 and CRABPII may be related to CP tumor recurrence and ATRA could be a potential therapeutic agent for CP chemotherapy.
ISSN:1878-8750
1878-8769
DOI:10.1016/j.wneu.2016.05.063