Experimental conical‐head abutment screws on the microbial leakage through the implant‐abutment interface: an in vitro analysis using target‐specific DNA probes

Objectives The aim of this in vitro study was to identify and quantify up to 38 microbial species from human saliva penetrating through the implant‐abutment interface in two different implant connections, external hexagon and tri‐channel internal connection, both with conventional flat‐head or exper...

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Bibliographic Details
Published in:Clinical oral implants research 2017-07, Vol.28 (7), p.e68-e75
Main Authors: Pita, Murillo S., Nascimento, Cássio, Santos, Carla G. P., Pires, Isabela M., Pedrazzi, Vinícius
Format: Article
Language:English
Subjects:
Dental Implant-Abutment Design
Dental Implants - microbiology
Dental Leakage
Dentistry
DNA Probes
Humans
microbiology
Nucleic Acid Hybridization
periodontology
prosthodontics
Saliva - microbiology
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Summary:Objectives The aim of this in vitro study was to identify and quantify up to 38 microbial species from human saliva penetrating through the implant‐abutment interface in two different implant connections, external hexagon and tri‐channel internal connection, both with conventional flat‐head or experimental conical‐head abutment screws. Material and methods Forty‐eight two‐part implants with external hexagon (EH; n = 24) or tri‐channel internal (TI; n = 24) connections were investigated. Abutments were attached to implants with conventional flat‐head or experimental conical‐head screws. After saliva incubation, Checkerboard DNA–DNA hybridization was used to identify and quantify up to 38 bacterial colonizing the internal parts of the implants. Kruskal–Wallis test followed by Bonferroni's post‐tests for multiple comparisons was used for statistical analysis. Results Twenty‐four of thirty‐eight species, including putative periodontal pathogens, were found colonizing the inner surfaces of both EH and TI implants. Peptostreptococcus anaerobios (P = 0.003), Prevotella melaninogenica (P 
ISSN:0905-7161
1600-0501
DOI:10.1111/clr.12876