Thiamine Deficiency Increases Ca2+ Current and CaV1.2 L-type Ca2+ Channel Levels in Cerebellum Granular Neurons

Thiamine (vitamin B1) is co-factor for three pivotal enzymes for glycolytic metabolism: pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, and transketolase. Thiamine deficiency leads to neurodegeneration of several brain regions, especially the cerebellum. In addition, several neurodegenerative...

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Bibliographic Details
Published in:Cellular and molecular neurobiology 2017-04, Vol.37 (3), p.453-460
Main Authors: Moreira-Lobo, Daniel C., Cruz, Jader S., Silva, Flavia R., Ribeiro, Fabíola M., Kushmerick, Christopher, Oliveira, Fernando A.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Calcium channels (L-type)
Calcium channels (voltage-gated)
Calcium metabolism
Calcium signalling
Cell Biology
Cerebellum
Dehydrogenases
Enzymes
Glycolysis
Granule cells
Ketoglutaric acid
Metabolism
Neurobiology
Neurodegeneration
Neurodegenerative diseases
Neurosciences
Original Research
Oxoglutarate dehydrogenase (lipoamide)
Pyruvic acid
Thiamine
Transketolase
Vitamin B
Vitamin deficiency
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Summary:Thiamine (vitamin B1) is co-factor for three pivotal enzymes for glycolytic metabolism: pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, and transketolase. Thiamine deficiency leads to neurodegeneration of several brain regions, especially the cerebellum. In addition, several neurodegenerative diseases are associated with impairments of glycolytic metabolism, including Alzheimer’s disease. Therefore, understanding the link between dysfunction of the glycolytic pathway and neuronal death will be an important step to comprehend the mechanism and progression of neuronal degeneration as well as the development of new treatment for neurodegenerative states. Here, using an in vitro model to study the effects of thiamine deficiency on cerebellum granule neurons, we show an increase in Ca 2+ current density and Ca V 1.2 expression. These results indicate a link between alterations in glycolytic metabolism and changes to Ca 2+ dynamics, two factors that have been implicated in neurodegeneration.
ISSN:0272-4340
1573-6830
DOI:10.1007/s10571-016-0378-8