Body mass index and the visceral adipose tissue expression of IL-6 and TNF-alpha are associated with the morphological severity of non-alcoholic fatty liver disease in individuals with class III obesity

Summary Objectives To analyze the mRNA expression of interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α) in the liver and white adipose tissue samples of individuals with class III obesity (body mass index ≥40.0 kg/m2 ) with non-alcoholic fatty liver disease (NAFLD). Methods This cross-sec...

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Published in:Obesity research & clinical practice 2018-01, Vol.12 (1), p.1-8
Main Authors: Jorge, Antônio Sérgio Barcala, Andrade, João Marcus Oliveira, Paraíso, Alanna Fernandes, Jorge, Gislaine Candida Batista, Silveira, Christine Mendes, de Souza, Ludmilla Regina, Santos, Erivelton Pereira, Guimaraes, André Luiz Sena, Santos, Sérgio Henrique Sousa, De-Paula, Alfredo Maurício Batista
Format: Article
Language:English
Subjects:
Adult
Anthropometric factors
Bariatric Surgery
Biomarkers - metabolism
Body Mass Index
Class III obesity
Cross-Sectional Studies
Endocrinology & Metabolism
Female
Gene Expression Profiling
Humans
Inflammation Mediators - metabolism
Interleukin-6 - metabolism
Internal Medicine
Intra-Abdominal Fat - metabolism
Male
Middle Aged
Non-alcoholic fatty liver disease
Non-alcoholic Fatty Liver Disease - complications
Non-alcoholic Fatty Liver Disease - metabolism
Non-alcoholic Fatty Liver Disease - physiopathology
Non-alcoholic Fatty Liver Disease - surgery
Obesity, Morbid - complications
Obesity, Morbid - metabolism
Obesity, Morbid - physiopathology
Obesity, Morbid - surgery
Proinflammatory cytokines
Real-time-PCR
Severity of Illness Index
Tumor Necrosis Factor-alpha - metabolism
Young Adult
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Summary:Summary Objectives To analyze the mRNA expression of interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α) in the liver and white adipose tissue samples of individuals with class III obesity (body mass index ≥40.0 kg/m2 ) with non-alcoholic fatty liver disease (NAFLD). Methods This cross-sectional study included patients with class III obesity exhibiting early or late morphological presentation of NAFLD (non-alcoholic hepatic steatosis [NAFL], n = 8 and non-alcoholic steatohepatitis [NASH], n = 13, respectively). All patients underwent bariatric surgery and peripheral blood, liver, and visceral white adipose tissue (WAT) samples were collected. Socio-demographic, anthropometric, clinical, plasma biochemical, and nutritional characteristics of each study subject were assessed and compared between patients presenting with NAFL and NASH. IL-6 and TNF-α mRNA expression in the liver and WAT samples were measured by using quantitative real time-polymerase chain reaction (qRT-PCR). Results Individuals with class III obesity and NASH showed higher body mass index (BMI) and higher IL-6 and TNF-α mRNA expression in the WAT compared to that of patients with NAFL ( p = 0.01, for all associations). Conclusions Individuals with class III obesity with higher morphological severity of NAFLD exhibited higher BMI and higher IL-6 and TNF-α expression in the WAT. Future prospective studies are warranted to determine how BMI, IL-6, and TNF-α affect the progression of NAFLD in individuals with class III obesity.
ISSN:1871-403X
1878-0318
DOI:10.1016/j.orcp.2016.03.009